IP Library Granted Patent US 9,546,363
Granted Patent B2
US 9,546,363 · App. 14/399,983 · Granted Jan 17, 2017

Method for stabilizing ascorbic acid oxidase

Inventors: Kenta Kinjo (Sunto-gun, JP); Tomoko Aratake (Sunto-gun, JP)
Assignee: KYOWA MEDEX CO., LTD.
C12N9/96C12N9/0063C12Y110/03003
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Quick Facts
Patent No.
US 9,546,363
App. No.
14/399,983
Granted
Jan 17, 2017
Kind
B2
Abstract

Provided are: a method for stabilizing an ascorbic acid oxidase; a method for preserving an ascorbic acid oxidase; and a stabilized composition of an ascorbic acid oxidase. A method for stabilizing an ascorbic acid oxidase and a method for preserving an ascorbic acid oxidase, each of the methods comprising allowing an ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium; and a stabilized composition of an ascorbic acid oxidase, which comprises an ascorbic acid oxidase being allowed to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium. The method for stabilizing an ascorbic acid oxidase, the method for preserving an ascorbic acid oxidase, and the stabilized composition of an ascorbic acid oxidase according to the present invention are useful for clinical diagnosis and the like.

Claims (6)

1. A method for stabilizing an ascorbic acid oxidase, which comprises allowing the ascorbic acid oxidase to coexist at −5 to 45° C. for at least 10 days with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium, wherein

the salt is selected from the group consisting of a lithium salt, a sodium salt, a potassium salt, an ammonium salt, a calcium salt and a magnesium salt, and

the nitrous acid ester is selected from the group consisting of methyl nitrite, ethyl nitrite, propyl nitrite, butyl nitrite, pentyl nitrite and isopentyl nitrite.

2. A method for preserving an ascorbic acid oxidase, which comprises allowing the ascorbic acid oxidase to coexist at −5 to 45° C. for at least 10 days with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium, wherein

the salt is selected from the group consisting of a lithium salt, a sodium salt, a potassium salt, an ammonium salt, a calcium salt and a magnesium salt, and

the nitrous acid ester is selected from the group consisting of methyl nitrite, ethyl nitrite, propyl nitrite, butyl nitrite, pentyl nitrite and isopentyl nitrite.

Assignments (4)
CHANGE OF NAME Recorded Sep 23, 2025
From: KYOWA MEDEX CO., LTD.
To: HITACHI CHEMICAL DIAGNOSTICS SYSTEMS CO., LTD.
Reel/Frame 072925/0508 →
CHANGE OF NAME Recorded Sep 23, 2025
From: HITACHI CHEMICAL DIAGNOSTICS SYSTEMS CO., LTD.
To: MINARIS MEDICAL CO., LTD.
Reel/Frame 072925/0657 →
CHANGE OF NAME Recorded Sep 23, 2025
From: MINARIS MEDICAL CO., LTD.
To: CANON MEDICAL DIAGNOSTICS CORPORATION
Reel/Frame 072925/0690 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 10, 2014
From: KINJO, KENTA; ARATAKE, TOMOKO
To: KYOWA MEDEX CO., LTD.
Reel/Frame 034194/0944 →
Priority Claims (1)
JP 2012-119582 · May 25, 2012 · national
Continuity (1)
Related Publication 20150147798A1 · May 28, 2015