IP Library › Granted Patent US 10,550,397
Granted Patent B2
US 10,550,397 · App. 14/401,109 · Granted Feb 4, 2020

Method for producing recombinant 11-de-O-methyltomaymycin

Inventors: Claus Lattemann (Frankfurt am Main, DE); Mark Broenstrup (Frankfurt am Main, DE); Stefan Werner (Saarbrücken, DE); Rolf Müller (Blieskastel, DE); Kirsten Harmrols (Saarbrücken, DE)
Assignee: SANOFI
C12N15/76C07K14/36
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Quick Facts
Patent No.
US 10,550,397
App. No.
14/401,109
Granted
Feb 4, 2020
Kind
B2
Abstract

The present invention provides a tomaymycin biosynthetic gene cluster of Streptomyces species FH6421, and its use for producing 11-de-O-methyltomaymycin.

Claims (10)

1. An expression vector comprising the gene cluster of SEQ ID NO: 2 or a variant or fragment thereof wherein the variant or fragment encodes proteins with at least 90% amino acid sequence identity to SEQ ID NOs: 4 to 22.

2. A cell comprising the expression vector according to claim 1 .

3. A method for producing a cell that harbors the expression vector of claim 1 , the method comprising transforming a cell with the expression vector.

4. The method according to claim 3 , wherein the cell harbors the tomaymycin biosynthetic gene cluster of SEQ ID NO: 2.

5. The method according to claim 4 , wherein the cell is a Streptomyces strain.

6. The method according to claim 5 , wherein the Streptomyces strain is selected from the group consisting of Streptomyces achromogenes var. tomaymyceticus, Streptomyces species FH6421, and Streptomyces albus /pStW102tc.

7. The method according to claim 3 , wherein the cell is capable of producing 11-de-O-methyltomaymycin.

8. The expression vector according to claim 1 , comprising the nucleic acid sequence of SEQ ID NO: 3.

9. The cell according to claim 2 , wherein the cell is a Streptomyces cell.

10. The cell according to claim 2 , wherein the cell is a Streptomyces albus cell.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 18, 2015
From: LATTEMANN, CLAUS; BROENSTRUP, MARK; WERNER, STEFAN; HARMROLS, KIRSTEN; MULLER, ROLF
To: SANOFI
Reel/Frame 035032/0733 →
Priority Claims (1)
EP 12305529 · May 14, 2012 · regional
Continuity (1)
Related Publication 20150111298A1 · Apr 23, 2015