Anti-BLyS antibody
The present invention belongs to the field of biopharmaceutics. Disclosed is an anti-BLyS antibody. The anti-BLyS antibody specifically targets BLyS, can combine with a B lymphocyte stimulating factor, and can inhibit the combination of the B lymphocyte stimulating factor with the receptor BR3-Fc thereof. Also provided are uses of the anti-BLyS antibody in the manufacture of a medicament for preventing and/or treating diseases caused by the excessive proliferation of B cells such as systemic lupus erythematorsus.
1. An anti-B lymphocyte Stimulator (anti-BlyS) antibody, wherein the amino acid sequences of the light chains CDR1, CDR2 and CDR3, and the amino acid sequences of the heavy chains CDR1, CDR2 and CDR3 are selected from one of the following groups:
(a) SEQ ID NOs: 1, 2, 3, 4, 5, and 6, respectively;
(b) SEQ ID NOs: 7, 8, 9, 10, 11, and 12, respectively;
(c) SEQ ID NOs: 13, 14, 15, 16, 17, and 18, respectively;
(d) SEQ ID NOs: 19, 20, 21, 22, 23, and 24, respectively; and
(e) SEQ ID NOs: 25, 26, 27, 28, 29, and 30, respectively.
2. The anti-BLyS antibody according to claim 1 , wherein the amino acid sequences of the light chain variable region and the amino acid sequences of the heavy chain variable region of the anti-BLyS antibody are selected from the group consisting of:
a: amino acid sequences as shown by SEQ ID NO: 31 and SEQ ID NO: 32;
b: amino acid sequences as shown by SEQ ID NO: 33 and SEQ ID NO: 34;
c: amino acid sequences as shown by SEQ ID NO: 35 and SEQ ID NO: 36;
d: amino acid sequences as shown by SEQ ID NO: 37 and SEQ ID NO: 38; and
e: amino acid sequences as shown by SEQ ID NO: 39 and SEQ ID NO: 40.
3. The anti-BLyS antibody according to claim 1 , wherein said antibody is humanized.
4. The anti-BLyS antibody according to claim 3 , wherein said antibody further comprises a human light chain constant region and a human heavy chain constant region, and the light chain variable region and the heavy chain variable region connect to the human light chain constant region and the human heavy chain constant region respectively.
5. The anti-BLyS antibody according to claim 4 , wherein the human light chain constant region is a human light chain κ constant region.
6. The anti-BLyS antibody according to claim 4 , wherein the human heavy chain constant region is a human heavy chain Fc fragment.
7. The anti-BLyS antibody according to claim 1 , wherein the amino acid sequences of the light chain variable region and the amino acid sequences of the heavy chain variable region of the anti-BLyS antibody are selected from the group consisting of:
I: amino acid sequences as shown by SEQ ID NO: 41 and SEQ ID NO: 42;
II: amino acid sequences as shown by SEQ ID NO: 43 and SEQ ID NO: 44;
III: amino acid sequences as shown by SEQ ID NO: 45 and SEQ ID NO: 46; and
IV: amino acid sequences as shown by SEQ ID NO: 47 and SEQ ID NO: 48.
8. A DNA molecule encoding the anti-BLyS antibody of claim 1 .
9. The DNA molecule according to claim 8 , wherein the DNA molecule has a nucleotide sequence selected from one of SEQ ID NOs: 49-58.
10. A recombinant DNA vector, comprising the DNA molecule of claim 8 .
11. A host cell, comprising the recombinant DNA vector of claim 10 .
12. A method for preparing an anti-BLyS antibody, comprising:
incubating the host cell of claim 11 , and obtaining the antibody.
13. A method for treating diseases caused by over proliferation of B cells in an individual in need thereof, comprising administrating to said individual an effective dosage of the anti-BLyS antibody of claim 1 .
14. The method according to claim 13 , wherein the diseases caused by over proliferation of B cells are selected from systemic lupus erythematosus, rheumatoid arthritis, ankylosing arthritis or B cell lymphoma.
15. A pharmaceutical composition, comprising an effective dosage of the antibody of claim 1 and a pharmaceutically acceptable carrier.