Biomarker test for prediction or early detection of preeclampsia and/or HELLP syndrome
Disclosed are specific biomarkers that allow for early testing of preeclampsia/HELLP syndrome. Thus, a method is provided predicting preeclampsia in a pregnant woman. Also disclosed is a kit comprising means for assaying a sample from a pregnant woman for the concentrations of the specific biomarkers.
1. A method for treating preeclampsia or HELLP syndrome, said method comprising:
administering an effective amount of an agent which prevents or reduces symptoms of preeclampsia or HELLP syndrome in a female and/or fetus subject, said administering taking place before said symptoms manifest in the female and/or fetus, wherein the subject has been identified by a method comprising:
obtaining a biological sample from a pregnant human female before the 15 th week of pregnancy;
determining levels of three or more biomarkers in the biological sample in a panel, wherein the panel comprises apolipoprotein H precursor and at least two other biomarkers selected from the group consisting of complement factor B, gelsolin isoform a precursor, hornerin, fetuin B, hemopexin precursor, fms-related tyrosine kinase 1, hydroxysteroid (17-β) dehydrogenase 1, leptin, lectin galactoside-binding soluble 14, pappalysin 2, and placenta-specific 1;
generating a dataset based on the determined levels; and
diagnosing the presence or risk of developing preeclampsia or HELLP syndrome in the subject based on a biostatistical analysis and comparison of the dataset to a reference dataset corresponding to known presence.
2. The method of claim 1 for treating preeclampsia wherein the panel comprises apolipoprotein H precursor and at least two other biomarkers selected from the group consisting of complement factor B, hornerin, hemopexin precursor, hydroxysteroid (17-β) dehydrogenase 1, lectin galactoside-binding soluble 14, and pappalysin 2; wherein the administering of the agent prevents or reduces symptoms of preeclampsia in the subject.
3. A method according to claim 1 , wherein the biological sample is a blood sample.
4. A method according to claim 1 , wherein the biological sample is a body fluid, secretion, or excretion sample other than blood.
5. A method according to claim 1 , wherein the biological sample is an amniotic fluid sample.
6. A method according to claim 1 , wherein the biological sample is fetal cells obtained invasively or non-invasively.
7. A method according to claim 1 , wherein the biological sample is a placental sample.
8. A method according to claim 1 , wherein the biological sample is obtained before the 14 th week of pregnancy, before the 13 th week of pregnancy, before the 12 th week of pregnancy, before the 11 th week of pregnancy, before the 10 th week of pregnancy, before the 9 th week of pregnancy, before the 8 th week of pregnancy, before the 7 th week of pregnancy, or before the 6 th week of pregnancy.
9. The method of claim 1 wherein the levels of the three or more biomarkers are determined by a protein-based assay method.
10. The method of claim 1 wherein the levels of the three or more biomarkers are determined by using specific antibodies produced against the biomarkers whereby the biological sample is contacted with the antibodies and binding between the biomarkers and the antibodies is detected.
11. The method of claim 1 wherein the accuracy of risk assessment is more than 75%.
12. A method according to claim 1 , wherein the biological sample is a cervicovaginal fluid, saliva, or urine sample.
13. A method according to claim 1 , said administering taking place before the 16 th week of pregnancy.