IP Library Granted Patent US 9,951,306
Granted Patent B2
US 9,951,306 · App. 14/407,772 · Granted Apr 24, 2018

Catalase in growth media

Inventors: Werner Besenmatter (Wiener Neudorf, AT); Axel Niebisch (Vienna, AT); Jan Modregger (Brunn am Gebirge, AT); Bhupinder Hundle (Worcestershire, GB)
Assignee: Eucodis Bioscience GmbH
C12N1/20C12N9/0065C12Q1/045C12Q1/18C12Q1/22C12Q1/30C12Y111/01006
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Quick Facts
Patent No.
US 9,951,306
App. No.
14/407,772
Granted
Apr 24, 2018
Kind
B2
Abstract

The present invention relates to the use of a secreted fungal catalase, for hydrogen-peroxide neutralization in growth media for the detection of microorganisms as well as to a method for detecting microorganisms in hydrogen peroxide-bearing aerosol or on a hydrogen peroxide bearing surface, said method comprising contacting said aerosol or surface with a growth medium comprising a secreted fungal catalase, and detecting growth of microorganisms in said medium.

Claims (13)

1. A hydrogen-peroxide neutralizing growth medium comprising at least 0.3 U/ml of a secreted fungal catalase in admixture with a growth medium, between 0.2 g/l and 4 g/l sodium pyruvate, and between 0.005 g/l and 0.05 g/l sodium thiosulfate, wherein the growth medium is a solid agar growth medium.

2. The medium according to claim 1 , wherein said medium is sterilized.

3. The medium according to claim 1 , wherein said catalase is derived from a species of the genus Magnaporthe or Scytalidium.

4. The medium according to claim 3 , wherein the catalase is derived from Magnaporthe grisea or from Scytalidium thermophilum.

5. The medium according to claim 1 , wherein the concentration of catalase in the growth medium is between 0.3 U/ml and 90 U/ml.

6. The medium according to claim 1 wherein the concentration of sodium pyruvate in the growth medium is between 0.2 g/l and 2 g/l.

7. A method for detecting microorganisms in a hydrogen peroxide-bearing aerosol or on a hydrogen peroxide-bearing surface, said method comprising contacting said aerosol or surface with a medium according to claim 1 and detecting growth of microorganisms in said medium.

8. The method according to claim 7 , comprising the steps of:

a. bringing a hydrogen peroxide-bearing aerosol or a hydrogen peroxide-bearing surface into contact with a growth medium comprising the catalase according to claim 1 ;

b. placing the growth medium in an environment allowing the development of colonies of microorganisms; and

c. determining if colonies of microorganisms which may have developed during step (b) are present.

9. The method according to claim 7 , wherein the hydrogen peroxide-bearing aerosol comprises air.

10. The method of claim 7 , further comprising the step of sterilizing the medium with gamma-radiation.

Assignments (4)
CHANGE OF NAME Recorded Jul 29, 2025
From: EUCODIS BIOSCIENCE GMBH
To: BIOSYNTH GMBH
Reel/Frame 072254/0387 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 5, 2015
From: BESENMATTER, WERNER
To: EUCODIS BIOSCIENCE GMBH
Reel/Frame 035841/0476 →
CORRECTIVE ASSIGNMENT TO CORRECT THE LAST NAME OF THIRD INVENTOR PREVIOUSLY RECORDED AT REEL: 034532 FRAME: 0615. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT. Recorded Jun 4, 2015
From: NIEBISCH, AXEL; MODREGGER, JAN; HUNDLE, BHUPINDER
To: EUCODIS BIOSCIENCE GMBH
Reel/Frame 035831/0809 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 17, 2014
From: NIEBISCH, AXEL; MODREGGER, JAN; MODREGGER, BHUPINDER
To: EUCODIS BIOSCIENCE GMBH
Reel/Frame 034532/0615 →
Priority Claims (1)
EP 12171798 · Jun 13, 2012 · regional
Continuity (1)
Related Publication 20150152466A1 · Jun 4, 2015