Cell-penetrating peptide, conjugate comprising same and composition comprising same
The present invention relates to a conjugate of cell penetrating peptide and an active ingredient; and its use. Specifically, a conjugate including a cell penetrating peptide which is a peptide comprising amino acid sequence of SEQ ID NO:1, a fragment of any one sequence of SEQ ID NO:1, or a peptide having above 80% homology with the above-mentioned sequence; and a composition comprising the same are disclosed.
1. A conjugate of a carrier peptide and an active ingredient,
wherein the carrier peptide is an isolated peptide 16 amino acids in length and consists of the amino acid sequence of SEQ ID NO: 1;
wherein the carrier peptide and the active ingredient are connected via a covalent bond, or via a linker;
and the active ingredient is not telomerase or a fragment thereof.
2. The conjugate according to claim 1 , wherein the active ingredient is a protein.
3. The conjugate according to claim 1 , wherein the active ingredient is connected to the C-terminus of the carrier peptide.
4. A composition comprising the conjugate according to claim 1 .
5. The composition according to claim 4 , wherein the active ingredient is for treatment of disease, and the composition is a pharmaceutical composition.
6. A mitochondria targeting delivery system for an active ingredient,
wherein the delivery system comprises the conjugate according to claim 1 , and
wherein the carrier peptide is a peptide that moves into a mitochondria locally and delivers the active ingredient to the mitochondria.
7. A composition for modulating mitochondria activity,
wherein the composition comprises the conjugate according to claim 1 ,
wherein the carrier peptide is a peptide that moves into a mitochondria locally and delivers the active ingredient to the mitochondria.
8. The conjugate according to claim 2 , wherein the protein is a cytokine, antibody, fragment of antibody, therapeutic enzyme, soluble receptor, or ligand.
9. The conjugate according to claim 2 , wherein the protein is an antibody or a fragment of antibody.
10. The conjugate according to claim 2 , wherein the protein is an enzyme or enzyme inhibitor.
11. The conjugate according to claim 2 , wherein the protein is a soluble receptor or ligand.
12. The conjugate according to claim 2 , wherein the protein is a vaccine.
13. The conjugate according to claim 2 , wherein the protein is a signal transfer protein.
14. The conjugate according to claim 2 , wherein the protein is a hormone or hormone analogue.
15. The conjugate according to claim 2 , wherein the linker is a Hynic linker.
16. The conjugate according to claim 1 , wherein more than one carrier peptide are connected to one active ingredient.