COCHLEATES MADE WITH SOY PHOSPHATIDYLSERINE
Unpurified or low pure soy phosphatidylserine is used to make cochleates. The cochleates contain about 40-74% soy phosphatidylserine, a multivalent cation and a biological active. A preferred cochleate contains the antifungal agent amphotericin B.
1 - 35 . (canceled)
36 . A crystal cochleate, comprising:
a soy-based phospholipid comprising 50% by weight soy phosphatidylserine,
calcium, and
amphotericin B.
37 . A geode cochleate, comprising:
a soy-based phospholipid that comprises 50% by weight soy phosphatidylserine,
calcium,
an oil, and
amphotericin B
38 . The crystal cochleate of claim 6 , wherein the ratio of the soy-based phospholipid to amphotericin B is about 5:1.
39 . The geode cochleate of claim 37 , wherein the ratio of the soy-based phospholipid to amphotericin B is about 5:1.
40 . A method of making the crystal cochleate of claim 36 , wherein the method comprises:
(a) dissolving amphotericin B in a sodium hydroxide solution to form a basic amphotericin B solution;
(b) mixing the basic amphotericin B solution and the soy-based phospholipid comprising 50% by weight soy phosphatidylserine to form liposomes in an aqueous solution, wherein the liposomes contain amphotericin B; and
(c) adding calcium to the liposomes to form the crystal cochleate.
41 . The method of claim 40 , wherein the ratio of the soy-based phospholipid to amphotericin B is about 5:1.
42 . The method of claim 40 , wherein the aqueous solution is a phosphate buffered solution.
43 . The method of claim 40 , further comprising adding an antioxidant to the liposomes before adding the calcium.
44 . The method of claim 43 , further comprising lyophilizing the crystal cochleate.
45 . A method of making the geode cochleate of claim 37 , wherein the method comprises:
(a) dissolving amphotericin B in a sodium hydroxide solution to form a basic amphotericin B solution;
(b) mixing the basic amphotericin B solution with the oil and the soy-based phospholipid comprising 50% by weight soy phosphatidylserine to form geode liposomes in an aqueous solution, wherein the geode liposomes contain amphotericin B; and
(c) adding calcium to the geode liposomes to form the geode cochleate.
46 . The method of claim 45 , wherein the ratio of the soy-based phospholipid to amphotericin B is about 5:1.
47 . The method of claim 45 , further comprising adding an antioxidant to the liposomes containing the amphotericin B before adding the calcium.
48 . The method of claim 45 , farther comprising adjusting the pH of the geode liposomes of step (b) or the geode cochleate of step (c) to neutral.
49 . The method of claim 45 , further comprising lyophilizing the geode cochleate.