PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS

1 . Process for preparing a sugar product from ligno-cellulosic material, comprising:

a) optionally pre-treatment of the ligno-cellulosic material;

b) optionally washing of the optionally pre-treated ligno-cellulosic material;

c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and

d) optionally recovery of a sugar product;

wherein after the pre-treatment and before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

2 . Process for preparing a fermentation product from ligno-cellulosic material, comprising:

a) optionally pre-treatment of the ligno-cellulosic material;

b) optionally washing of the optionally pre-treated ligno-cellulosic material;

c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61;

d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and

e) optionally recovery of a fermentation product;

wherein after the pre-treatment and before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

3 . Process according to claim 1 , wherein during the enzymatic hydrolysis c) oxygen is added to the ligno-cellulosic material.

4 . Process according to claim 1 , wherein the oxygen is added in the form of bubbles.

5 . Process according to claim 1 , wherein the reactor for the enzymatic hydrolysis has a volume of 1 m 3 or more.

6 . Process according to claim 2 , wherein the enzymatic hydrolysis time is 5 to 150 hours.

7 . Process according to claim 1 , wherein the enzyme composition used retains activity for 30 hours or more.

8 . Process according to claim 1 , wherein the hydrolysis is conducted at a temperature of 45° C. or more, optionally 50° C. or more and optionally at a temperature of 55° C. or more.

9 . Process according to claim 1 , wherein the

enzyme composition is derived from a fungus, optionally microorganism of the genus Rasamsonia or the enzyme composition comprises a fungal enzyme, a Rasamsonia enzyme.

10 . Process according to claim 1 , wherein the dry matter content in the hydrolysis c) is 10 wt % or more, optionally is 14 wt % or more and optionally is 14 to 33 wt %.

11 . Process according to claim 9 , wherein the dry matter content in the hydrolysis c) is 14 to 33% wt %.

12 . A process according to claim 1 in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.

13 . A process according to claim 1 in which oxygen is introduced as an oxygen-containing gas optionally air.