Observation apparatus and optical axis adjustment method
Ordinary charged particle beam apparatuses have each been an apparatus manufactured for dedicated use in making observations in a gas atmosphere at atmospheric pressure or at a pressure substantially equal thereto. There have existed no devices capable of simply making observations using an ordinary high-vacuum charged particle microscope in a gas atmosphere at atmospheric pressure or at a pressure approximately equal thereto. Furthermore, ordinary techniques have been incapable of observing the same spot of the sample in such an atmosphere using a charged particle beam and light simultaneously. This invention thus provides an apparatus including: a charged particle optical tube that irradiates a sample with a primary charged particle beam; a vacuum pump that evacuates the inside of the charged particle optical tube; a diaphragm arranged to separate a space in which the sample is placed from the charged particle optical tube, the diaphragm being detachable and allowing the primary charged particle beam to permeate or pass therethrough; and an optical microscope positioned on the opposite side of the charged particle optical tube across the diaphragm and the sample, the optical microscope having an optical axis thereof aligned with at least part of an extension of the optical axis of the charged particle optical tube.
1. An observation apparatus comprising:
a charged particle optical tube that irradiates a sample with a primary charged particle beam;
a vacuum pump that evacuates the inside of the charged particle optical tube;
a diaphragm arranged to remain out of contact with the sample and to separate a space in which the sample is placed from the charged particle optical tube, the diaphragm being detachable and allowing the primary charged particle beam to permeate or pass therethrough;
an optical microscope positioned on the opposite side of the charged particle optical tube across the diaphragm and the sample, the optical microscope having an optical axis thereof aligned with at least part of an extension of the optical axis of the charged particle optical tube; and
an optical axis adjustment mechanism configured to adjust and fix a position of the optical axis of the optical microscope so that the center of the diaphragm is positioned on the optical axis of the optical microscope.
2. The observation apparatus according to claim 1 , wherein the optical axis adjustment mechanism can move the optical microscope independently of the charged particle optical tube and the diaphragm.
3. The observation apparatus according to claim 1 , further comprising a stage, wherein the stage can be moved independently of the optical microscope, the charged particle optical tube, and the diaphragm.
4. The observation apparatus according to claim 1 , further comprising:
a first enclosure that supports the charged particle beam apparatus as a whole against an apparatus installation surface, the first enclosure having the inside thereof evacuated by the vacuum pump; and
a second enclosure of which the position is fixed to a side or an inner wall surface of the first enclosure or to the charged particle optical tube, the second enclosure holding the sample thereinside, wherein:
the diaphragm is located on the upper surface side of the second enclosure; and
the pressure inside the second enclosure is kept equal to or higher than the pressure inside the first enclosure.
5. The observation apparatus according to claim 4 , further comprising an adjustment mechanism that moves the position of the diaphragm, wherein at least part of the drive mechanism is located inside the second enclosure.
6. The observation apparatus according to claim 4 , further comprising a sample stage interposed between the charged particle optical tube and the optical microscope, the sample stage having the sample placed thereon, wherein the sample stage is located inside the second enclosure.
7. The observation apparatus according to claim 4 , further comprising a signal formation unit that converts image information acquired by the optical microscope into a digital signal, the signal formation unit being installed inside the second enclosure.
8. The observation apparatus according to claim 7 , further comprising:
a cover part that can cover at least one side of the second enclosure; and
a signal output port or a signal line lead-out port that outputs the signal from the optical microscope to the outside of the second enclosure, the port being furnished in the cover part.
9. The observation apparatus according to claim 4 , further comprising a mechanism that operates the sample stage and a mechanism that operates movement of the optical microscope, the mechanisms being attached to a cover part that can cover at least one side of the second enclosure.
10. The observation apparatus according to claim 4 , wherein:
at least part of the optical microscope is located inside the second enclosure; and
an observation unit for observing an image from the optical microscope is located outside the second enclosure.
11. The observation apparatus according to claim 4 , further comprising a vacuum pump that can bring about a vacuum state inside the second enclosure.
12. The observation apparatus according to claim 1 , wherein the space in which the sample is placed is an atmosphere having pressures ranging from 10 3 Pa to atmospheric pressure inclusive.
13. The observation apparatus according to claim 1 , further comprising a gas introduction port that can replace the atmosphere at least in a space between the diaphragm and the sample with a gas other than air.
14. The observation apparatus according to claim 1 , further comprising a drive mechanism that moves the position of optical lenses belonging to the optical microscope in the direction of the optical axis of the optical microscope.
15. An optical axis adjustment method for use with an observation apparatus having an optical microscope and a charged particle beam microscope including a charged particle optical tube, the observation apparatus permitting observation of an image from the charged particle beam microscope and an image from the optical microscope, the images being acquired of a sample placed in an atmosphere having pressures ranging from 10 3 Pa to atmospheric pressure inclusive, the optical axis adjustment method comprising:
a diaphragm position adjustment step for adjusting the position of a diaphragm arranged to separate a space in which the sample is placed from the charged particle optical tube so that the center of the diaphragm is positioned on an extension of the optical axis of the charged particle beam microscope; and
after the diaphragm position adjustment step above, a step for adjusting the position of the optical microscope, with the position of the diaphragm being fixed, so that the diaphragm center is positioned on an extension of the optical axis of the optical microscope.
16. An observation method for use with an observation apparatus having an optical microscope and a charged particle beam microscope including a charged particle optical tube, the observation apparatus permitting observation of an image from the charged particle beam microscope and an image from the optical microscope, the images being acquired of a sample placed in an atmosphere having pressures ranging from 10 3 Pa to atmospheric pressure inclusive, the optical axis adjustment method comprising:
a diaphragm position adjustment step for adjusting the position of a diaphragm arranged to separate a space in which the sample is placed from the charged particle optical tube so that the center of the diaphragm is positioned on an extension of the optical axis of the charged particle beam microscope;
after the diaphragm position adjustment step, an optical microscope adjustment step for adjusting the position of the optical microscope, with the position of the diaphragm being fixed, so that the diaphragm center is positioned on an extension of the optical axis of the optical microscope;
after the optical microscope adjustment step, a focusing step for positioning a focus of the charged particle beam microscope and a focus of the optical microscope to a point close to the diaphragm; and
after the focusing step, a moving step for moving the sample to the point close to the diaphragm.