Medium for yeasts
View Patent ↗In order to provide a culture medium eliminating variation by lot of component concentrations, the present invention provides a culture medium, and preferably a chemical-synthetic culture medium, imparting a proliferation ability to yeast that is equivalent to or greater than that of a YPD culture medium. A culture medium is provided which includes sugars as a carbon source capable of being assimilated by yeast; amino acids as a nitrogen source; vitamins; inositol; zinc ion (Zn 2+ ); potassium ion (K + ), and magnesium ion (Mg 2+ ), and in which inositol concentration is between 50 and 10,000 mg/L.
1. A yeast culture medium comprising:
a) a sugar capable of being assimilated by yeast;
b) an amino acid comprising aspartic acid, acetyl tyrosine, or proline;
c) vitamins;
d) inositol;
e) zinc ions (Zn 2+ );
f) potassium ions (K + ), and
g) magnesium ions (Mg 2+ ), and
wherein:
the amount of sugar is between 1 g/L and 200 g/L;
the amount of aspartic acid is between 0.1 g/L and 10 g/L, when selected;
the amount of proline is between 0.1 g/L and 5 g/L, when selected;
the amount of acetyl tyrosine is between 0.1 g/L and 5 g/L, when selected;
the amount of zinc ions is between 0.405 μg/L and 2024.9 μg/L;
the amount of potassium ions is between 0.0287 g/L and 2.87 g/L;
the amount of magnesium ions is between 0.986 mg/L and 493 mg/L; and
the amount of inositol is between 50 mg/L and 10,000 mg/L.
2. The yeast culture medium according to claim 1 further comprising adenine, uracil, guanine, cytosine, or thymine.
3. The yeast culture medium according to claim 1 further comprising at least two amino acids selected from arginine, lysine, proline, glutamic acid, alanine, isoleucine, phenylalanine, valine, methionine, serine, threonine, glycine, asparagine, glutamine, cysteine, leucine, tryptophan, and histidine.
4. The yeast culture medium according to claim 1 , wherein ammonium ions are not present in the medium.
5. The yeast culture medium according to claim 1 , wherein the vitamins comprise at least two selected from biotin, pantothenic acid, niacin, pyridoxine, and thiamine.
6. The yeast culture medium according to claim 1 , wherein the sugar comprises glucose, lactose, mannose, fructose, sucrose, maltose, or raffinose.
7. The yeast culture medium according to claim 1 , wherein yeast extract is not present in the medium.
8. The yeast culture medium according to claim 1 , wherein the vitamins comprises between 0.02 μg/L and 10 μg/L of biotin.
9. A yeast culture medium comprising the following the concentrations of structural components
TABLE 1
Structural
Concen-
Concen-
Component
tration
Structural Component
tration
Glucose
20
g/L
Asparagine
0.5
g/L
KH 2 PO 4
1
g/L
Glutamine
5
g/L
MgSO 4 •7H 2 O
0.5
g/L
Cysteine
0.01
g/L
Aspartic acid
5
g/L
Leucine
1
g/L
Arginine
10
g/L
Tryptophan
0.1
g/L
Lysine
1
g/L
Histidine
0.1
g/L
Proline
1
g/L
Adenine
0.1
g/L
Glutamic acid
1
g/L
Uracil
0.1
g/L
Alanine
1
g/L
myo-Inositol
1
g/L
Isoleucine
1
g/L
Pantothenic acid
4000
μg/L
Phenylalanine
1
g/L
Nicotinic acid (Niacin)
4000
μg/L
Valine
1
g/L
Pyridoxine
400
μg/L
N-Acetyl tyrosine
1
g/L
Thiamine
400
μg/L
Methionine
0.1
g/L
Biotin
2
μg/L
Serine
0.5
g/L
Ferric (III) chloride
4000
μg/L
Threonine
0.5
g/L
Zinc sulfate
400
μg/L
Glycine
0.1
g/L.
10. A method of determining nutritional requirements of a yeast comprising:
growing a yeast under aerobic conditions in the yeast culture medium of claim 9 ,
growing the yeast under aerobic conditions in a modified yeast culture medium of claim 9 from which one structural component has been eliminated and/or reduced in concentration, and
determining whether the elimination and/or reduction of the structural component affects the growth of the yeast.
11. A method of determining nutritional requirements of a yeast comprising:
growing a yeast under aerobic conditions in the yeast culture medium of claim 9 ,
growing the yeast under aerobic conditions in a first modified yeast culture medium of claim 9 from which a first structural component has been eliminated and/or reduced in concentration,
determining whether the elimination and/or reduction of the first structural component affects the growth of the yeast,
growing the yeast under aerobic conditions in a second modified yeast culture medium of claim 9 from which a second structural component has been eliminated or reduced in concentration, and
determining whether the elimination and/or reduction of the second structural component affects the growth of the yeast.
12. A method of determining nutritional requirements of a yeast comprising:
growing a yeast under aerobic conditions in the yeast culture medium of claim 9 ,
growing the yeast under aerobic conditions in a first modified yeast culture medium of claim 9 from which a first structural component has been eliminated and/or reduced in concentration,
growing the yeast under aerobic conditions in a second modified yeast culture medium of claim 9 from which a second structural component has been eliminated or reduced in concentration,
determining whether the elimination and/or reduction of the first structural component affects the growth of the yeast,
determining whether the elimination and/or reduction of the second structural component affects the growth of the yeast, and
ranking the first structural component and second structural component by the growth of the yeast.