Methods for producing high toughness silk fibres
The present invention provides methods for producing a silk protein spinning dope solution suitable for producing high toughness fibers, the thus produced silk protein spinning dope solution, methods for producing fibers using said silk protein spinning dope solution.
1. Fibre comprising at least one silk protein, wherein at least 10% by weight of the fibre is (are) silk protein(s), wherein the silk protein monomer(s) has(have) a molecular weight in the range of 20 kDa to 600 kDa and the fibre has a toughness (MJ/m 3 ) that is the product of the molecular weight of the silk protein(s) in kDa and the factor of at least 1.0 to a molecular weight of the silk protein(s) of 300 kDa and is at least 300 MJ/m 3 for proteins with a molecular weight of above 300 kDa;
wherein the silk protein comprises at least two repetitive units each comprising at least one consensus sequence selected from the group consisting of:
(a) GPGXX (SEQ ID NO: 3), wherein X is in each case independently selected from the amino acids A, S, G, Y, P, and Q;
(b) GGX, wherein X is in each case independently selected from the amino acids Y, P, R, S, A, T, N and Q; and
(c) A x , wherein A is an amino acid and x is an integer from 5 to 10; and,
wherein the repetitive units are selected from the group consisting of module A (SEQ ID NO: 20) or a variant thereof, module C (SEQ ID NO: 21) or a variant thereof, and module Q (SEQ ID NO: 22) or a variant thereof;
wherein the variant of module A (SEQ ID NO: 20) has a sequence identity of at least 90% to said module, the variant of module C (SEQ ID NO: 21) has a sequence identity of at least 90% to said module, and the variant of module Q (SEQ ID NO: 22) has a sequence identity of at least 90% to said module.
2. The fibre of claim 1 , wherein the silk protein further comprises at least one non-repetitive (NR) unit.
3. The fibre of claim 2 , wherein the NR unit is NR3 (SEQ ID NO: 41), NR4 (SEQ ID NO: 42), NR5 (SEQ ID NO: 45) or NR6 (SEQ ID NO: 46) or variants thereof.
4. The fibre of claim 1 , wherein the fibre further comprises a synthetic or natural polymer.
5. The fibre of claim 4 , wherein the polymer is polyamide, polycaprolactone, polyacrylate, polyaramide, polylactic acid (PLA), polypropylene, polylacetate, polyhydroxybutyrate, polyurethane, xanthan, cellulose, collagen, tropoelastin, elastin, keratin, cotton, wool or mixtures thereof.
6. The fibre of claim 1 , wherein the at least two repetitive units are module C (SEQ ID NO: 21) or a variant thereof,
wherein the variant of module C (SEQ ID NO: 21) has a sequence identity of at least 90% to said module.
7. The fibre of claim 1 , wherein the at least two repetitive units are module A (SEQ ID NO: 20) or a variant thereof and module Q (SEQ ID NO: 22) or a variant thereof,
wherein module A (SEQ ID NO: 20) or a variant thereof is combined with module Q (SEQ ID NO: 22) or a variant thereof, and
wherein the variant of module A (SEQ ID NO: 20) has a sequence identity of at least 90% to said module and the variant of module Q (SEQ ID NO: 22) has a sequence identity of at least 90% to said module.