IP Library › Granted Patent US 9,968,885
Granted Patent B2
US 9,968,885 · App. 14/439,218 · Granted May 15, 2018

Techniques for CO

Inventors: Richard Daigle (Lévis, CA); Éric Madore (Québec, CA); Sylvie Fradette (Lévis, CA)
Assignee: CO2 Solutions Inc.
B01D53/84B01D53/1475B01D53/1493C12M21/18C12M29/20C12N9/88B01D2252/20494B01D2252/602B01D2255/804C12Y402/01001Y02C10/04Y02C10/06Y02P20/59
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Quick Facts
Patent No.
US 9,968,885
App. No.
14/439,218
Granted
May 15, 2018
Kind
B2
Abstract

Use of Sulfurihydrogenibium sp. carbonic anhydrase (SspCA) or mutants thereof for catalyzing the hydration reaction of CO 2 into bicarbonate and hydrogen ions or catalyzing the desorption reaction to produce a CO 2 gas is provided.

Claims (56)

1. A recombinant carbonic anhydrase polypeptide having carbonic anhydrase activity comprising an amino acid sequence having at least 85% identity to SEQ ID NO: 8 and one or more differences as compared to SEQ ID NO: 8 at residue positions selected from 18, 20, 38, 52, 57, 82, 100, 130, 150, and 181.

2. The recombinant carbonic anhydrase polypeptide of claim 1 , comprising one or more amino acid differences as compared to SEQ ID NO: 8 selected from:

(a) 18A, 18C, 18F, 18L, 18R, 18S, 18T, or 18W;

(b) 20A, 20G, 20L, 20N, 20R, 20S, 20T, or 20W;

(c) 38A, 38D, 38G, 38L, 38N, 38P, 38R, 38S, or 38W;

(d) 52C, 52E, 52G, 52P, or 52T;

(e) 57A, 57G, 57L, 57N, 57P, 57R, 57S, or 57V;

(f) 82C or 82E;

(g) 100A, 100E, 100N, 100S, 100V, or 100Y;

(h) 130A, 130C, or 130L;

(i) 150A, 150I, 150N, or 150S; and

(j) 181Q, 181L, 181M, or 181R.

3. The recombinant carbonic anhydrase polypeptide of claim 2 , further comprising an amino acid difference as compared to SEQ ID NO: 8 selected from 14D, 65S, 88E, 114I, 116D, 122I, 126L, 148A, 155I, and 205C.

4. The recombinant carbonic anhydrase polypeptide of claim 1 , having at least 90% identity to SEQ ID NO: 8.

5. The recombinant carbonic anhydrase polypeptide of claim 1 , having at least 95% identity to SEQ ID NO: 8.

6. The recombinant carbonic anhydrase polypeptide of claim 1 comprising two or more amino acid differences as compared to SEQ ID NO: 8 which are: 18T and 20A; 18R and 20A; 2K, 181M, and 1971; 14D and 18R; 52C, 122I, 150N, and 226S; 65S and 150I; 57R and 130C; 82C and 88E; 82C and 148A; 126L and 130L; 82C and 100V; 38C, 82C, and 100V; 38G, 82C, and 100V; 38R, 82C, and 100V; 38S, 82C, and 100V; 38W, 82C, and 100V; 38S, 57A, 82C, and 100V; 38S, 57G, 82C, and 100V; 38S, 57L, 82C, and 100V; 38S, 57S, 82C, and 100V; 38S, 57V, 82C, and 100V; 18F, 20G, 38S, 57L, 82C, and 100V; 18R, 20G, 38S, 57L, 82C, and 100V; 18W, 20G, 38S, 57L, 82C, and 100V; 18R, 20W, 38S, 57L, 82C, and 100V; 18R, 20A, 38S, 57L, 82C, and 100V; 18R, 20R, 38S, 57L, 82C, and 100V; 18C, 20S, 38S, 57L, 82C, and 100V; 18C, 20V, 38S, 57L, 82C, and 100V; 18A, 20T, 38S, 57L, 82C, and 100V; or 18F, 20R, 38S, 57L, 82C, and 100V.

7. A recombinant carbonic anhydrase polypeptide having carbonic anhydrase activity comprising:

(a) an amino acid sequence having at least 80% identity to SEQ ID NO: 8; and

(b) one or more differences as compared to SEQ ID NO: 8 at residue positions selected from 18, 20, 38, 52, 57, 82, 100, 130, 150, and 181,

wherein said recombinant carbonic anhydrase polypeptide has improved stability relative to the carbonic anhydrase of SEQ ID NO: 8, following 15 minutes of exposure at 92° C. in 0.3M Na 2 CO 3 /NaHCO 3 pH 10.

8. The recombinant carbonic anhydrase polypeptide claim 7 , comprising one or more amino acid differences as compared to SEQ ID NO: 8 selected from:

(a) 18A, 18C, 18F, 18L, 18R, 18S, 18T, or 18W;

(b) 20A, 20G, 20L, 20N, 20R, 20S, 20T, or 20W;

(c) 38A, 38D, 38G, 38L, 38N, 38P, 38R, 38S, or 38W;

(d) 52C, 52E, 52G, 52P, or 52T;

(e) 57A, 57G, 57L, 57N, 57P, 57R, 57S, or 57V;

(f) 82C or 82E;

(g) 100A, 100E, 100N, 100S, 100V, or 100Y;

(h) 130A, 130C, or 130L;

(i) 150A, 150I, 150N, or 150S; and

(j) 181Q, 181L, 181M, or 181R.

9. The recombinant carbonic anhydrase polypeptide claim 8 , further comprising an amino acid difference as compared to SEQ ID NO: 8 selected from 14D, 65S, 88E, 114I, 116D, 122I, 126L, 148A, 155I, and 205C.

10. The recombinant carbonic anhydrase polypeptide claim 7 , having at least 85% identity to SEQ ID NO: 8.

11. The recombinant carbonic anhydrase polypeptide claim 7 , having at least 90% identity to SEQ ID NO: 8.

12. The recombinant carbonic anhydrase polypeptide claim 7 , having at least 95% identity to SEQ ID NO: 8.

13. A recombinant carbonic anhydrase polypeptide having carbonic anhydrase activity comprising

(1) an amino acid sequence having at least 75% identity to SEQ ID NO: 8;

(2) an amino acid difference as compared to SEQ ID NO: 8 which is 82C; and

(3) one or more further amino acid differences as compared to SEQ ID NO: 8 selected from:

(a) 18A, 18C, 18F, 18L, 18R, 18S, 18T, or 18W;

(b) 20A, 20G, 20L, 20N, 20R, 20S, 20T, or 20W,

(c) 38A, 38D, 38G, 38L, 38N, 38P, 38R, 38S, or 38W,

(d) 52C, 52E, 52G, 52P, or 52T,

(e) 57A, 57G, 57L, 57N, 57P, 57R, 57S, or 57V,

(f) 100A, 100E, 100N, 100S, 100V, or 100Y,

(g) 130A, 130C, or 130L,

(h) 150A, 150I, 150N, or 150S; and

(i) 181Q, 181L, 181M, or 181R,

wherein said recombinant carbonic anhydrase polypeptide has improved stability relative to the carbonic anhydrase of SEQ ID NO: 8, following 15 minutes of exposure at 92° C. in 0.3M Na 2 CO 3 /NaHCO 3 pH 10.

14. The recombinant carbonic anhydrase polypeptide of claim 2 , having at least 90% identity to SEQ ID NO: 8.

15. The recombinant carbonic anhydrase polypeptide of claim 2 , having at least 95% identity to SEQ ID NO: 8.

16. The recombinant carbonic anhydrase polypeptide of claim 7 comprising two or more amino acid differences as compared to SEQ ID NO: 8 which are: 18T and 20A; 18R and 20A; 2K, 181M, and 1971; 14D and 18R; 52C, 122I, 150N, and 226S; 65S and 150I; 57R and 130C; 82C and 88E; 82C and 148A; 126L and 130L; 82C and 100V; 38C, 82C, and 100V; 38G, 82C, and 100V; 38R, 82C, and 100V; 38S, 82C, and 100V; 38W, 82C, and 100V; 38S, 57A, 82C, and 100V; 38S, 57G, 82C, and 100V; 38S, 57L, 82C, and 100V; 38S, 57S, 82C, and 100V; 38S, 57V, 82C, and 100V; 18F, 20G, 38S, 57L, 82C, and 100V; 18R, 20G, 38S, 57L, 82C, and 100V; 18W, 20G, 38S, 57L, 82C, and 100V; 18R, 20W, 38S, 57L, 82C, and 100V; 18R, 20A, 38S, 57L, 82C, and 100V; 18R, 20R, 38S, 57L, 82C, and 100V; 18C, 20S, 38S, 57L, 82C, and 100V; 18C, 20V, 38S, 57L, 82C, and 100V; 18A, 20T, 38S, 57L, 82C, and 100V; or 18F, 20R, 38S, 57L, 82C, and 100V.

17. The recombinant carbonic anhydrase polypeptide of claim 8 , having at least 85% identity to SEQ ID NO: 8.

18. The recombinant carbonic anhydrase polypeptide of claim 8 , having at least 90% identity to SEQ ID NO: 8.

19. The recombinant carbonic anhydrase polypeptide of claim 13 , further comprising an amino acid difference as compared to SEQ ID NO: 8 selected from 14D, 65S, 88E, 114I, 116D, 122I, 126L, 148A, 155I, and 205C.

20. The recombinant carbonic anhydrase polypeptide of claim 13 comprising two or more amino acid differences as compared to SEQ ID NO: 8 which are: 18T and 20A; 18R and 20A; 2K, 181M, and 1971; 14D and 18R; 52C, 122I, 150N, and 226S; 65S and 150I; 57R and 130C; 82C and 88E; 82C and 148A; 126L and 130L; 82C and 100V; 38C, 82C, and 100V; 38G, 82C, and 100V; 38R, 82C, and 100V; 38S, 82C, and 100V; 38W, 82C, and 100V; 38S, 57A, 82C, and 100V; 38S, 57G, 82C, and 100V; 38S, 57L, 82C, and 100V; 38S, 57S, 82C, and 100V; 38S, 57V, 82C, and 100V; 18F, 20G, 38S, 57L, 82C, and 100V; 18R, 20G, 38S, 57L, 82C, and 100V; 18W, 20G, 38S, 57L, 82C, and 100V; 18R, 20W, 38S, 57L, 82C, and 100V; 18R, 20A, 38S, 57L, 82C, and 100V; 18R, 20R, 38S, 57L, 82C, and 100V; 18C, 20S, 38S, 57L, 82C, and 100V; 18C, 20V, 38S, 57L, 82C, and 100V; 18A, 20T, 38S, 57L, 82C, and 100V; or 18F, 20R, 38S, 57L, 82C, and 100V.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 10, 2020
From: CO2 SOLUTIONS INC.
To: SAIPEM S.P.A
Reel/Frame 054157/0632 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 29, 2015
From: DAIGLE, RICHARD; MADORE, ÉRIC; FRADETTE, SYLVIE
To: CO2 SOLUTIONS INC.
Reel/Frame 035530/0047 →
Continuity (2)
Provisional Application 61719710 · Oct 29, 2012
Related Publication 20150283502A1 · Oct 8, 2015