Fertility gene and use thereof
The present disclosure relates to a fertility gene and the use thereof, and relates to the biotechnology field, particularly to a method of plant hybrid breeding including creation of a sterile line and preparation of hybrid seeds, more particularly to a fertility gene FL2, a mutant thereof and use thereof in hybrid breeding.
1. A method for producing a male sterile Oryza sativa plant, comprising the step of:
inactivating the FL2 gene in an Oryza sativa plant, wherein the FL2 gene encodes the cDNA sequence of SEQ ID NO:1, or
inhibiting the expression of the FL2 gene in the Oryza sativa plant, thereby producing a male sterile Oryza sativa plant.
2. The method of claim 1 , further comprising:
restoring male sterility in the plant by breeding.
3. The method of claim 2 , wherein the breeding comprises:
hybridizing the plant as a female parent sterile line with a restorer line to produce a hybrid seed.
4. The method of claim 1 , wherein the step of inactivating the FL2 gene in an Oryza sativa plant comprises introducing a G→A mutation at position 1688 of SEQ ID NO:1.