Modulation of muscle and adipocyte distribution and fate
View Patent ↗Modulation of the ADAMTS1 signaling pathway alters the commitment of progenitor cells to a muscle fate; and increases muscle mass.
1. A method of enhancing myogenesis, the method comprising:
contacting a population of human muscle satellite cells in vivo in an individual suffering from a myopathy with an effective dose of a polypeptide consisting of SEQ ID NO:4, wherein the dose is effective to increase muscle mass or to reduce loss of muscle mass in the individual.
2. A method of enhancing myogenesis, the method comprising:
administering to an individual suffering from a myopathy an effective dose of a purified active fragment of human ADAMTS1 protein comprising SEQ ID NO:1, residues 258-463; thereby contacting a population of human muscle satellite cells, wherein the dose is effective to increase muscle mass or to reduce loss of muscle mass in the individual.
3. The method of claim 2 , wherein the active fragment of human ADAMTS1 protein comprises the polypeptide of SEQ ID NO:4.
4. The method of claim 3 , wherein the active fragment lacks ADAMTS1 C-terminal portion from residues 725-967.
5. The method of claim 2 , wherein the active fragment is administered by intramuscular injection.
6. The method of claim 5 , wherein the active fragment consists of the polypeptide of SEQ ID NO:4.
7. The method of claim 1 or claim 2 , wherein the myopathy consists of sarcopenia.