In vitro test system to evaluate xenobiotics as immune-modulators of drug transport and metabolism in human hepatocytes
A kit and method for evaluating in vitro the effect of a xenobiotic (e.g., a biologic drug) on drug metabolism in hepatocytes. The kit comprises a first culture of hepatocytes, a portion of in vitro xenobiotic-stimulated biological sample, and instructions for incubating the first culture of hepatocytes with the portion of in vitro xenobiotic-stimulated biological sample, and analyzing the activity, expression, or a combination thereof of a biomarker in the hepatocytes to evaluate the effect of the xenobiotic on drug metabolism in the hepatocytes.
1. A kit for evaluating in vitro the effect of a xenobiotic on drug metabolism in hepatocytes, said kit comprising:
cryopreserved hepatocytes;
an in vitro xenobiotic-stimulated biological sample, wherein said biological sample has been stimulated in vitro by said xenobiotic for about 12 to about 48 hours to yield said in vitro xenobiotic-stimulated biological sample, wherein said biological sample is selected from the group consisting of whole blood, bone marrow, and isolated peripheral blood mononuclear cells, wherein said xenobiotic is a small molecule or biologic drug; and
instructions for incubating said hepatocytes with said in vitro xenobiotic-stimulated biological sample and analyzing the activity, expression, or a combination thereof of a biomarker in said hepatocytes to evaluate the effect of said xenobiotic on drug metabolism in said hepatocytes.
2. The kit of claim 1 , wherein said cryopreserved hepatocytes further comprise Kupffer cells.
3. The kit of claim 1 , wherein said hepatocytes are human hepatocytes.
4. The kit of claim 1 , wherein said hepatocytes are pooled hepatocytes.
5. The kit of claim 4 , wherein said pooled hepatocytes comprise a combination of cells from different genders, genotypes, ages, races, enzymatic or metabolic activities, species, or disease or health states.
6. The kit of claim 1 , wherein said in vitro xenobiotic-stimulated biological sample is a portion of in vitro xenobiotic-stimulated biological sample prepared by exposing a first culture of said biological sample to said xenobiotic in vitro to yield a xenobiotic-stimulated biological sample, and separating said portion of said xenobiotic-stimulated biological sample.
7. The kit of claim 6 , wherein said portion of in vitro xenobiotic-stimulated biological sample is plasma that has been separated from said in vitro xenobiotic-stimulated whole blood.
8. The kit of claim 6 , wherein said portion of in vitro xenobiotic-stimulated biological sample is a cytokine-containing fraction that has been separated from said in vitro xenobiotic-stimulated bone marrow.
9. The kit of claim 6 , wherein said portion of in vitro xenobiotic-stimulated biological sample is a cytokine-containing fraction that has been separated from said in vitro xenobiotic-stimulated peripheral blood mononuclear cells.
10. The kit of claim 1 , wherein said xenobiotic is a biologic drug.
11. The kit of claim 10 , wherein said biologic drug is selected from the group consisting of hormones, growth factors, cytokines, receptors, antibodies, enzymes, oligonucleotides, aptamers, synthetic peptides, and vaccines.
12. The kit of claim 1 , wherein said in vitro xenobiotic-stimulated biological sample further comprises an anticoagulant.