Granted Patent B2

US 9,382,551 · App. 14/460,838 · Granted Jul 5, 2016

Inventors: Soumitra Roy (Noordwijk, NL); James M. Wilson (Glen Mills, PA)

Assignee: The Trustees of the University of Pennsylvania

C12N15/86A61K35/761C07K14/005C12N7/00A61K48/00C12N2710/10021C12N2710/10022C12N2710/10041C12N2710/10043

Abstract

Claims (40)

1. An adenovirus having a capsid comprising a hexon protein, a penton protein, and a fiber protein, wherein said hexon protein is the hexon protein of SAdV-A1295 with the amino acids 1 to 940 of SEQ ID NO: 67, and said fiber protein is the fiber protein of SAdV-A1295 with the amino acids 1 to 442 of SEQ ID NO: 78;

said capsid encapsidating a heterologous nucleic acid comprising a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.

2. The adenovirus according to claim 1 , further comprising a 5′ and a 3′ adenovirus cis-element necessary for replication and encapsidation.

3. The adenovirus according to claim 1 , wherein said adenovirus lacks all or a part of the E1 gene.

4. The adenovirus according to claim 3 , wherein said adenovirus is replication—defective.

5. The adenovirus according to claim 1 , wherein said penton protein is the penton protein of SAdV-A1295 with the amino acids 1 to 534 of SEQ ID NO: 62.

6. The adenovirus according to claim 1 , wherein said capsid is a hybrid capsid.

7. The adenovirus according to claim 6 , wherein said hybrid capsid comprises at least one capsid protein from an adenovirus selected from SAdV-A1321, SAdV-A1325, SAdV-A1316, and SAdV-1322.

8. A composition comprising the adenovirus according to claim 1 in a pharmaceutically acceptable carrier.

9. A composition comprising the adenovirus according to claim 1 , said adenovirus further comprising at least one simian adenovirus protein selected from the group consisting of:

E1a, SEQ ID NO: 85;

E1b, small T/19K, SEQ ID NO: 58;

E1b, large T/55K, SEQ ID NO: 80;

IX, SEQ ID NO: 59;

52/55D, SEQ ID NO: 60;

IIIa, SEQ ID NO: 61;

Penton, SEQ ID NO: 62;

VII, SEQ ID NO: 63;

V, SEQ ID NO: 64;

pX, SEQ ID NO: 65;

VI, SEQ ID NO: 66;

Endoprotease, SEQ ID NO: 68;

100 kD, SEQ ID NO: 69;

22 kD, SEQ ID NO: 81;

VIII, SEQ ID NO: 70;

E3/12.5 K, SEQ ID NO: 71;

CR1-alpha SEQ ID NO: 82;

gp19K, SEQ ID NO: 72;

CR1-beta, SEQ ID NO: 73;

CR1-gamma, SEQ ID NO: 74;

CR1-delta, SEQ ID NO: 75;

RID-alpha, SEQ ID NO: 76

RID-beta, SEQ ID NO: 77;

E3/14.7K, SEQ ID NO: 83; and

Fiber, SEQ ID NO: 78.

10. A vector comprising at least one simian adenovirus nucleic acid sequence selected from the group consisting of:

(a) the open reading frame for the penton of SAdV-A1295, nucleotides 13857 to 15458 of SEQ ID NO: 57;

(b) the open reading frame for the hexon of SAdV-A1295, nucleotides 18290 to 21109 of SEQ ID NO: 57; and

(c) the open reading frame for the fiber protein of SAdV-A1295, nucleotides 32227 to 33552 of SEQ ID NO: 57;

said vector further comprising a heterologous gene operably linked to expression control sequences.

Continuity (9)