Rice resistant to HPPD and accase inhibiting herbicides
Rice is described that is tolerant/resistant to a plurality of herbicides, for example, ACCase and HPPD inhibitors. Use of the rice for weed control and methods of producing tolerant/resistant rice are also described.
1. A mutant rice plant resistant to both HPPD and ACCase inhibiting herbicides, wherein said mutant rice plant is produced from seeds deposited in the ATCC under Accession numbers PTA-121398 or PTA-121362.
2. The mutant rice plant of claim 1 , wherein the HPPD inhibiting herbicides are selected from the group consisting of mesotrione, benzobicyclon, and combinations thereof.
3. The mutant rice plant of claim 1 , wherein the HPPD inhibiting herbicides are selected from the group consisting of topramezone, tembotrione, isoxaflutole, and combinations thereof.
4. An F1 progeny rice plant of the mutant rice plant of claim 1 , or a part thereof, wherein the F1 progeny rice plant is resistant to both HPPD and ACCase inhibiting herbicides.
5. A tissue culture produced from protoplasts or cells from the mutant rice plant of claim 1 , wherein said cells or protoplasts of the tissue culture are produced from a plant part selected from the group consisting of leaves, pollen, embryos, cotyledon, hypocotyl, meristematic cells, roots, root tips, pistils, anthers, flowers, stems, glumes, and panicles.
6. A rice plant regenerated from the tissue culture of claim 5 , wherein the plant has all the morphological and physiological characteristics of the mutant rice plant of claim 1 .
7. A method for controlling weeds in a rice field, the method comprising:
(a) having the mutant rice plant of claim 1 , or the F1 progeny rice plant of claim 5 in the field, wherein the mutant rice plant or the F1 progeny rice plant is resistant to a plurality of herbicides; and
(b) contacting the rice field with at least one of the plurality of the herbicides to which the mutant rice plant is resistant at levels known to kill weeds wherein the plurality of herbicides comprise herbicides selected from the group consisting of ACCase and HPPD inhibitors.
8. The method of claim 7 , wherein the mutant rice plant or the F1 progeny rice plant is resistant to both ACCase and HPPD inhibitors.
9. The method of claim 7 , wherein the ACCase inhibiting herbicides are selected from the group consisting of aryloxyphenoxy propionate, fluazifop and quizalofop.
10. A method to develop a rice plant resistant to a plurality of herbicides comprising HPPD and ACCase inhibitors, the method comprising:
(a) introgressing genetic material derived from ATCC deposited lines PTA-13620 and PTA-12933 into rice lines through breeding, tissue culture or transformation, wherein said genetic material causes resistance to HPPD and ACCase inhibitors;
(b) selecting progeny of the introgressed lines that demonstrate the plurality of resistance,: and
(c) producing inbred or hybrid plants from the progeny.
11. The method of claim 10 , further comprising application of mesotrione at a rate of at least 420-630 gm ai/ha and quizalofop at a rate of at least 154 gm ai/ha and up to 210 gm ai/ha, to the rice plant resistant to the plurality of herbicides comprising HPPD and ACCase inhibitors.
12. A method of isolating a DNA molecule with a mutation associated with tolerance to mesotrione or other HPPD inhibiting herbicides, said method comprising isolating a DNA sequence from seeds deposited in the ATCC under Accession number PTA-13620, wherein the mutation is linked to, or located between, SNP markers with nucleic acid sequences selected from the group consisting of SEQ ID NO: 4 and SEQ ID NO: 5, the mutation causing tolerance to mesotrione or other HPPD inhibiting herbicides.
13. A recombinant nucleic acid comprising the mutant DNA molecule of claim 12 .
14. A mutant rice plant resistant to both HPPD and ACCase inhibiting herbicides, said mutant rice comprising the mutant DNA molecule of claim 12 and an ACCase tolerance mutation G2096S.