Granted Patent B2

US 10,022,721 · App. 14/470,860 · Granted Jul 17, 2018

Inventors: Sepehr Kiani (Watertown, MA); Joshua Blouwolff (Brookline, MA); Adnan Esmail (Boston, MA); Jason Hung (Arlington, MA); Tony Hung (Cambridge, MA); Adam R. Abate (San Francisco, CA); Scott Powers (Cambridge, MA); Pascaline Mary (Cambridge, MA)

Assignee: Bio-Rad Laboratories, Inc.

B01L3/502784B01L3/50273B01L3/502738C12N15/1075B01L7/525B01L2200/025B01L2200/0621B01L2200/0684B01L2200/10B01L2200/16B01L2300/021B01L2300/0645B01L2300/0654B01L2300/0672B01L2300/087B01L2300/0864B01L2300/0867B01L2300/0887B01L2400/0487B01L2400/0655B01L2400/0683B01L2400/0688B01L2400/084

Abstract

Claims (19)

1. A system for labeling and alternating flow of differentially-labeled droplets, the system comprising:

at least one inlet for droplets within a continuous phase,

at least two microfluidic channels that are connected to the at least one inlet at one end and form a junction with a joint microfluidic channel at the other end,

a connection channel configured to allow the continuous phase fluid but not the droplets to flow between the microfluidic channels, wherein the connection channel is sized such that the droplets are too large to enter one of the microfluidic channels, and wherein the connection channel is configured to equalize pressure between the two microfluidic channels, and

a labeling device configured to label droplets in one of the microfluidic channels;

wherein when the droplets flow through the at least two microfluidic channels toward the joint microfluidic channel, the labeling device labels the droplets that flow in one of the microfluidic channels.

2. The system of claim 1 , wherein said label is an immiscible label.

3. The system of claim 1 , wherein the labeling device labels the droplets through disrupting the interface between the immiscible label and the continuous phase.

4. The system of claim 3 , wherein the labeling device uses an electric field to disrupt the interface between the immiscible label and the continuous phase.

5. The system of claim 3 , wherein the labeling device comprises one or more electrodes that generate an electric field to disrupt the interface between the immiscible label and the continuous phase.

6. The system of claim 3 , wherein the droplets in the microfluidic channel is spaced so that neighboring droplets do not coalesce due to the presence of the electric field.

7. A method for labeling droplets and alternating flow of differentially-labeled droplets in the system of claim 1 , the method comprising the steps of:

injecting a plurality of droplets within a continuous phase into the at least one inlet, and

labeling the droplets in one of the microfluidic channels with a label using the labeling device, wherein labeled and unlabeled droplets enter the joint microfluidic channel in a substantially alternating fashion.

8. The method of claim 7 , wherein said label is an immiscible label.

9. The method of claim 7 , wherein the droplets contain primers and reagents that are necessary for a desired PCR reaction.

10. The method of claim 7 , wherein the labeling device labels the droplets through disrupting the interface between the immiscible label and the continuous phase.

11. The method of claim 10 , wherein the labeling device comprises one or more electrodes that generate an electric field to disrupt the interface between the immiscible label and the continuous phase.

12. The method of claim 11 , wherein the droplets in the microfluidic channel is spaced so that neighboring droplets do not coalesce due to the presence of the electric field.

Assignments (3)

Continuity (8)