The disclosure relates in one aspect to a fast acidifying lactic acid bacterium that generates a viscosity in fermented milk greater than about 62 Pa·s after 14 days of storage at 6° C. Further, the disclosure includes method for preparing and products of food, food additive, feed, nutritional supplement, or probiotic supplement, thereof.
1. A method for preparing a food, food additive, feed, nutritional supplement, or probiotic supplement, wherein:
the method comprises adding a fast acidifying Streptococcus thermophilus lactic acid bacterium,
the Streptococcus thermophilus lactic acid bacterium comprises the sequence set forth in SEQ ID No. 19 or a homologue thereof with at least 90% identity thereto or a fragment comprising at least 90% of the sequence set forth in SEQ ID No. 19,
the Streptococcus thermophilus lactic acid bacterium further comprises an EPS operon as set forth in in SEQ ID No. 20 or a homologue thereof with at least 99% identity thereto, and
the Streptococcus thermophilus lactic acid bacterium generates a viscosity in fermented milk greater than about 62 pascal-seconds (Pa·s) after 14 days of storage at 6° C.
2. The method according to claim 1 , wherein said lactic acid bacterium generates a viscosity in fermented milk greater than about 65 pascal-seconds (Pa·s) after 14 days of storage at 6° C.
3. The method according to claim 1 , wherein said lactic acid bacterium further comprises the sequence set forth in SEQ ID No.20.
4. The method according to claim 1 , wherein said lactic acid bacterium comprises a homologue of SEQ ID No. 19 with at least 95% identity thereto.
5. The method according to claim 1 , wherein said lactic acid bacterium is the Streptococcus thermophilus strain deposited at DSMZ (Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, Mascheroder Weg 1 b, D -38124Braunschweig) under deposit number 18344 on 14 Jun. 2006 or a mutant thereof and/or a variant thereof, wherein said mutant or variant generates a viscosity in fermented milk greater than about 62 pascal-seconds (Pa·s) after 14 days of storage at 6° C., wherein said mutant or variant comprises the sequence set forth in SEQ ID No. 19 or a homologue thereof with at least 90% identity thereto or a fragment comprising at least 90% of the sequence set forth in SEQ ID No. 19.
6. The method according to claim 1 , wherein said food is a fermented milk product, and wherein said fast acidifying Streptococcus thermophilus lactic acid bacterium is added to a milk substrate before said milk substrate is fermented.
7. The method according to claim 1 , wherein said lactic acid bacterium is added as a pure culture.
8. The method according to claim 1 , wherein said lactic acid bacterium is added as a mixed culture.
9. The method according to claim 8 , wherein said mixed culture comprises one or more further lactic acid bacteria selected from the genera consisting of Streptococcus, Lactococcus, Lactobacillus, Leuconostoc, Pediococcus and Bifidobacterium.
10. The method according to claim 9 , wherein said mixed culture comprises one or more further lactic acid bacteria selected from the species consisting of Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus, Lactobacillus casei and/or a Bifidobacterium sp.
11. The method according to claim 1 , wherein said lactic acid bacterium is the Streptococcus thermophilus strain deposited at DSMZ (Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, Mascheroder Weg 1 b, D -38124 Braunschweig) under deposit number 18344 on 14 Jun. 2006.
12. The method according to claim 1 , wherein said lactic acid bacterium comprises a homologue of SEQ ID No. 19 with at least 96% identity thereto.
13. The method according to claim 1 , wherein said lactic acid bacterium comprises a homologue of SEQ ID No. 19 with at least 97% identity thereto.
14. The method according to claim 1 , wherein said lactic acid bacterium comprises a homologue of SEQ ID No. 19 with at least 98% identity thereto.
15. The method according to claim 1 , wherein said lactic acid bacterium comprises a homologue of SEQ ID No. 19 with at least 99% identity thereto.
16. The method according to claim 1 , wherein said lactic acid bacterium comprises SEQ ID No. 19.
17. A food, food additive, feed, nutritional supplement, or probiotic supplement obtained or obtainable by the method of claim 1 .
18. A food, food additive, feed, nutritional supplement, or probiotic supplement according to claim 17 , wherein the food, food additive, feed, nutritional supplement, or probiotic supplement is a dairy, meat or cereal food, food additive, feed, nutritional supplement, or probiotic supplement.
19. A dairy food, food additive, feed, nutritional supplement, or probiotic supplement according to claim 18 , wherein the dairy food, food additive, feed, nutritional supplement, or probiotic supplement is a fermented milk, yoghurt, cream, matured cream, cheese, fromage frais, a milk beverage, a processed cheese, a cream dessert, a cottage cheese or infant milk.
20. A fermented milk product obtained or obtainable by the method of claim 6 .