Gray leaf spot tolerant maize and methods of production
View Patent ↗The invention relates to methods and compositions for identifying maize plants that have newly conferred tolerance or enhanced tolerance to, or are susceptible to, Gray Leaf Spot (GLS). The methods use molecular genetic markers to identify, select and/or construct tolerant plants or identify and counter-select susceptible plants. Maize plants that display newly conferred tolerance or enhanced tolerance to GLS that are generated by the methods of the invention are also a feature of the invention.
1. A method of identifying and selecting a maize plant that shows tolerance to gray leaf spot, said method comprising:
a. detecting in a maize plant at a locus linked to any of the following markers: PHM 15534-13, PHM 04694-10, PHM 01811-32, PHM 01963-15, PHM 01963-22, PHM 05013-12, and PHM 00586-10, an allele associated with one or more of the following:
i. an A at PHM 15534-13;
ii. a G at PHM 04694-10;
iii. a T at PHM 01811-32;
iv. a T at PHM 01963-15;
v. a C at PHM 01963-22;
vi. a T at PHM 05013-12; and
vii. a T at PHM 00586-10;
b. selecting a maize plant that has the allele associated with one or more of i-vii; and
c. crossing the maize plant to a second maize plant to transmit the allele associated with one or more of i-vii to at least one progeny.
2. The method of claim 1 , wherein the locus is linked to any of PHM 15534-13, PHM 04694-10, PHM 01811-32, PHM 01963-15, PHM01963-22, PHM 05013-12, and PHM 00586-10 by 25 cM on an IBM2 map.
3. The method of claim 1 , wherein the locus is linked to any of PHM 15534-13, PHM 04694-10, PHM 01811-32, PHM 01963-15, PHM01963-22, PHM 05013-12, and PHM 00586-10 by 10 cM on an IBM2 map.
4. The method of claim 1 , wherein the locus is linked to any of PHM 15534-13, PHM 04694-10, PHM 01811-32, PHM 01963-15, PHM 01963-22, PHM 05013-12, and PHM 00586-10 by 5 cM on an IBM2 map.
5. A method of identifying and selecting a maize plant that shows tolerance to gray leaf spot, said method comprising:
a. detecting in a maize plant one or more of the following:
i. an A at PHM 15534-13;
ii. a G at PHM 04694-10;
iii. a Tat PHM 01811-32;
iv. a Tat PHM 01963-15;
v. a C at PHM 01963-22;
vi. a Tat PHM 05013-12; and
vii. a Tat PHM 00586-10; and
b. selecting a maize plant that has one or more of i-vii; and
c. crossing the maize plant to a second maize plant to transmit one or more of i-vii to at least one progeny.
6. A method of making a progeny maize plant with tolerance to gray leaf spot, the method comprising:
a. obtaining a first parent maize plant that comprises within its genome one or more marker alleles selected from the group consisting of:
i. an A at PHM 15534-13;
ii. a G at PHM 04694-10;
iii. a Tat PHM 01811-32;
iv. a Tat PHM 01963-15;
v. a C at PHM 01963-22;
vi. a Tat PHM 05013-12; and
vii. a Tat PHM 00586-10;
b. crossing said first parent maize plant to a second parent maize plant;
c. growing the parent maize plant used as a female under plant growth conditions to yield maize plant progeny;
d. assaying the maize plant progeny for the presence of any of the marker alleles set forth in i-vii in step (a) or the presence of an allele linked to any of the marker alleles set forth in i-vii in step (a), wherein the marker alleles set forth in i-vii of step (a) or an allele linked to any of the marker alleles set forth in i-vii of step (a) are associated with tolerance to gray leaf spot; and
e. selecting progeny plants that possess any of the marker alleles set forth in i-vii in step (a) or an allele linked to any of the marker alleles set forth in i-vii in step (a).