Methods and compositions for obtaining useful plant traits
The present invention provides methods for obtaining plants that exhibit useful traits by perturbation of plastid function in plant rootstocks and grafting the rootstocks to scions. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that provide for plastid perturbation are also provided.
1. A grafted plant comprising a scion to which a rootstock had been grafted, wherein:
(i) the scion is from a wild type plant;
(ii) MSH1 gene expression is suppressed in the rootstock;
(iii) the rootstock confers an improvement in yield or growth rate in progeny of the grafted plant in comparison to a control plant, wherein the control plant comprises either: (a) progeny of a scion grafted to rootstock that had not been subjected to suppression of MSH1 gene expression; (b) a whole plant that lacks any root graft and that had not been subjected to suppression of MSH1 gene expression; (c) a wild-type plant; or (d) progeny of a plant that is isogenic to the plant source of the scion of the grafted plant; and,
(iv) the MSH1 gene expression is suppressed in the rootstock by a mutation in an endogenous MSH1 gene of the rootstock or by a small inhibitory RNA (siRNA), a microRNA (miRNA), a co-suppressing sense RNA, and/or an anti-sense RNA having complementarity to the endogenous MSH1 gene promoter, 5′ or 3′ untranslated region, intron, coding region, and/or any combination thereof.
2. The grafted plant of claim 1 , wherein MSH1 gene expression is suppressed in the rootstock and the rootstock confers to the grafted plant an improvement in yield or growth rate in comparison to a control plant.
3. The grafted plant of claim 1 , wherein the scion contains one or more epigenetic changes in one or more nuclear chromosomes, wherein the epigenetic changes are absent from the nuclear chromosomes of a control plant or are absent from nuclear chromosomes of a plant from which the scion was obtained.
4. The grafted plant of claim 3 , wherein the epigenetic change(s) are also present in the rootstock.
5. The grafted plant of claim 3 , wherein the epigenetic changes are associated with the improvement in the useful trait.
6. The grafted plant of claim 3 , wherein the rootstock contain(s) one or more epigenetic changes in one or more nuclear chromosomes that are absent from nuclear chromosomes of rootstock obtained from a plant or nuclear chromosomes of a parent plant thereof that had not been subjected to suppression of MSH1 gene expression.
7. The grafted plant of claim 3 , wherein the scion and/or the rootstock exhibit CG hypermethylation of a region encompassing a MSHI locus in comparison to a control plant that had not been subjected to suppression of MSH1 gene expression.
8. The grafted plant of claim 3 , wherein the scion and/or the rootstock exhibit pericentromeric CHG hyper-methylation in comparison to a control plant that had not been subjected to suppression of MSH1 gene expression.
9. The grafted plant of claim 3 , wherein the scion and/or the rootstock exhibit CG hypermethylation and/or CHG hypermethylation at one or more nuclear chromosomal loci in comparison to corresponding nuclear chromosomal loci of a control plant that had not been subjected to suppression of MSH1 gene expression.
10. The grafted plant of claim 1 , where said plant is selected from the group consisting of a crop plant, a tree, a bush, turf grass, pasture grass, and a vine.
11. The grafted plant of claim 10 , wherein the crop plant is selected from the group consisting of corn, soybean, cotton, canola, wheat, rice, tomato, tobacco, millet, potato, sugarbeet, cassava, alfalfa, barley, oats, sugarcane, sunflower, strawberry, and sorghum.
12. A progeny plant produced by a method comprising the steps of:
(a) obtaining a population of progeny plants from the grafted plant of claim 1 , wherein the population of progeny plants is a first, second, or third generation of progeny plants obtained by selfing the grafted plant or by selfing the first or second generation progeny plants;
(b) screening the population of progeny plants for plants having improved yield or growth rate in comparison to control plants; and
(c) selecting a progeny plant from the population for an improvement in yield or growth rate in comparison to a control plant, wherein said progeny plant exhibits said improvement in yield or growth rate and exhibits a nuclear chromosomal DNA methylation pattern that is distinct from the control plant nuclear chromosomal DNA methylation pattern, and wherein the control plant is grown under the same environmental conditions as the selected progeny plants and comprises either: (i) progeny of a scion grafted to rootstock that had not been subjected to suppression of MSH1 gene expression; (ii) a whole plant that lacks any root graft and that had not been subjected to suppression of MSH1 gene expression; (iii) a wild-type plant; or (iv) progeny of a plant that is isogenic to the plant source of the scion of the grafted plant.
13. A selected population of progeny plants produced by a method comprising the steps of:
(a) obtaining a population of progeny plants from the grafted plant of claim 1 , wherein the population of progeny plants is a first, second, or third generation of progeny plants obtained by selfing the grafted plant or by selfing the first or second generation progeny plants;
(b) screening the population of progeny plants for improved yield or growth rate in comparison to a control plant population; and
(c) selecting a population of progeny plants for an improvement in yield or growth rate in comparison to control plants, wherein said selected population of progeny plants exhibits said improvement in yield or growth rate and exhibits a nuclear chromosomal DNA methylation pattern that is distinct from a control plant nuclear chromosomal DNA methylation pattern, and wherein the control plant is grown under the same environmental conditions as the selected population of progeny plants and comprises either: (i) progeny of a scion grafted to rootstock that had not been subjected to suppression of MSH1 gene expression; (ii) a whole plant that lacks any root graft and that had not been subjected to suppression of MSH1 gene expression; (iii) a wild-type plant; or (iv) progeny of a plant that is isogenic to the plant source of the scion of the grafted plant.
14. The grafted plant of claim 1 , wherein the MSH1 gene expression is suppressed in the rootstock of the grafted plant by a mutation in an endogenous MSH1 gene of the rootstock.