Patent
Plants, plant parts and plant seeds that are resistant to imidazolinone herbicides are provided. Plants are disclosed that contain a mutation in an AHAS gene. Specifically, plants are disclosed that contain a mutant AHAS gene allele of the Brassica juncea B genome. Two B. juncea AHAS gene sequences (BjAHAS-a and BjAHAS-b) and one B. nigra AHAS gene sequence (BngrAHAS) are disclosed. The sequence of the mutant allele, BjAHAS-bR, is also disclosed. Various methods are disclosed that include creation of mutant B. juncea lines, selection for herbicide resistant lines and determining the presence of the BjAHAS-bR mutant allele after crosses.
1. A method for identifying a plant or seed comprising a mutagenized Brassica juncea acetohydroxyacid synthase (AHAS) nucleic acid encoding an herbicide resistance polypeptide comprising an amino acid sequence of residues 114 to 655 of SEQ ID NO:15, said method comprising:
(A) providing biological material from a plant obtained by a process comprising crossing a plant of line J04E-0044, a representative sample of seed of the line having been deposited under ATCC Accession No. PTA-6324, with another Brassica variety, or seed thereof,
(B) performing PCR or hybridization testing of an AHASL gene in said biological material to determine if the biological material comprises the nucleic acid encoding the herbicide resistance polypeptide, and
(C) identifying, based on the results of step (B), that the plant or seed of step (A) comprises said nucleic acid.
2. The method of claim 1 , wherein said biological material is a seed.
3. The method of claim 1 , wherein the nucleic acid encodes an herbicide resistance polypeptide comprising the sequence of SEQ ID NO:15.
4. The method of claim 3 , wherein the nucleic acid encodes an herbicide resistance polypeptide consisting of the sequence of SEQ ID NO:15.
5. The method of claim 1 , wherein the nucleic acid comprises the sequence of nucleotides 396 to 2024 of SEQ ID NO:14.
6. The method of claim 1 , wherein the nucleic acid comprises the sequence of SEQ ID NO:14.
7. The method of claim 6 , wherein the nucleic acid consists of the sequence of SEQ ID NO:14.
8. The method of claim 1 , wherein the nucleic acid is a Brassica juncea B-genome AHAS nucleic acid.
9. The method of claim 8 , wherein the plant or seed is a Brassica juncea plant or seed and the nucleic acid is located at the AHAS-b locus of the plant or seed.
10. A method for identifying a plant or seed comprising a mutagenized Brassica juncea acetohydroxyacid synthase (AHAS) nucleic acid comprising a sequence having at least 95% homology to SEQ ID NO:14 and encoding an herbicide resistance polypeptide that comprises the Ser to Asn substitution encoded by nucleotides 1968-1970 of SEQ ID NO:14, said method comprising:
(A) providing biological material from a plant obtained by a process comprising crossing a plant of line J04E-0044, a representative sample of seed of the line having been deposited under ATCC Accession No. PTA-6324, with another Brassica variety, or seed thereof,
(B) performing PCR or hybridization testing of an AHASL gene in said biological material to determine if the biological material comprises the nucleic acid encoding the herbicide resistance polypeptide, and
(C) identifying, based on the results of step (B), that the plant or seed of step (A) comprises said nucleic acid.
11. The method of claim 10 , wherein said biological material is a seed.
12. The method of claim 10 , wherein the nucleic acid is a Brassica juncea B-genome AHAS nucleic acid.
13. The method of claim 12 , wherein the plant or seed is a Brassica juncea plant or seed and the nucleic acid is located at the AHAS-b locus of the plant or seed.