Inhibition of DNA2 in fanconi anemia
Inhibition of DNA2 in Fanconi anemia (FA) cells remedies the over-resection of DNA, thereby stabilizing the FA cells. Inhibition of DNA2 in FA cells allows for safe treatment of cancers in FA patients, a decrease in the lethality of FA cells, a decrease in bone marrow failure of FA patients, and a means for decreasing the incidence of cancer for FA patients.
1. A method of decreasing lethality of human Fanconi anemia cells or human Fanconi anemia-like cells, comprising inhibiting a human DNA2 gene, a human DNA2 transcript, and/or a human DNA2 protein, the inhibiting comprising:
using a viral vector to disrupt the DNA2 gene; and/or
using interfering RNA (RNAi) to inhibit translation of the DNA2 transcript; and/or
using acetylation and/or phosphorylation to inhibit post-translational modification of the DNA2 protein in the Fanconi anemia cells or the Fanconi anemia-like cells.
2. The method of claim 1 , wherein the inhibiting the DNA2 transcript comprises interfering RNA (RNAi) directed against the DNA2 transcript.
3. The method of claim 1 , wherein the Fanconi anemia cells or the Fanconi anemia-like cells are in vitro or in vivo.
4. The method of claim 1 , wherein the inhibiting comprises:
using the viral vector to disrupt the DNA2 gene; and/or
using the interfering RNA (RNAi) to inhibit translation of the DNA2 transcript.
5. The method of claim 1 , wherein the disruption of the DNA2 gene comprises deletion of exon 2.
6. The method of claim 1 , wherein the RNAi is shorthairpin RNA (shRNA).
7. The method of claim 6 , wherein the shRNA is targeted to exon 22 of the DNA2 gene.