Cell binding peptides for diagnosis and detection
Cell binding peptides are provided for binding to cells including urothelial and thyroid follicular cells. The peptides are useful for detection and diagnosis of cancer including bladder and thyroid cancer. A device and method for using the device for capturing cells is provided, the device includes a support having attached cell binding peptide. The support can be a slide and the device can be used for detection and diagnosis of cancer including bladder and thyroid cancer. A kit is provided with instructions for capturing cells and a support with attached cell binding peptide for detection and diagnosis of bladder and thyroid cancer.
1. A device for capturing cells, the device comprising a support having an attached cell binding peptide for binding to and capturing cells, wherein the peptide comprises a sequence as set forth in:
a. SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, or SEQ ID NO: 10;
b. a conservatively substituted variant having at least 70% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, or SEQ ID NO: 10; or
c. a variant having at least 70% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, or SEQ ID NO: 10.
2. The device of claim 1 , wherein the support comprises glass, metal, plastic, synthetic matrix, silica gel, polymer, or derivatives or combinations thereof.
3. The device of claim 1 , wherein the support is in the form of slides, beads, magnetic beads, microtiter plates, cell culture plates, mesh, fibrous form, hollow fibers, or sheets.
4. The device of claim 1 , wherein the cell binding peptide comprises one or more modifications to the peptide N-terminus, peptide C-terminus, or within the peptide amino acid sequence, wherein the modification is one or more of acetyl group, aldehyde group, hydroxyl group, thiol group, amino group, biotin, amino acids, lysine, cysteine, glycine-serine-serine-glycine (GSSG; SEQ ID NO: 11), polymers, synthetic polymers, polyethers, poly(ethylene glycol) (“PEG”), an 11 unit polyethylene glycol (“PEG10”), a 1 unit polyethylene glycol (“mini-PEG” or “MP”), or combinations thereof.
5. The device of claim 4 , wherein one or both of an amine group of the peptide N-terminus and an internal lysine amine group of the peptide comprises the acetyl group modification.
6. The device of claim 4 , wherein the peptide C-terminus comprises the modification PEG10 linked to lysine, mini-PEG linked to lysine, GSSG (SEQ ID NO: 11)linked to lysine, or GSSG (SEQ ID NO: 11) linked to cysteine.
7. The device of claim 1 , wherein the support is in the form of a slide.
8. The device of claim 7 , wherein the slide is a N-Hydroxysuccinimide (NHS)-derivatized glass slide, and wherein the cell binding peptide is attached to the slide through a bond between a C-terminal lysine on the peptide and the NHS group on the slide.
9. The device of claim 7 , wherein the slide is an epoxy-derivatized glass slide, and wherein the cell binding peptide is attached to the slide through a bond between a C-terminal cysteine on the peptide and the epoxy group on the slide.
10. The device of claim 7 , wherein the slide is for evaluation of the captured cells for one or both of bladder cancer and thyroid cancer.
11. A method for capturing cells, the method comprising:
contacting a sample from a subject that comprises cells with the device of claim 1 having the support with attached cell binding peptide to bind to and capture cells, wherein the contacting is performed under conditions such that the cells are captured onto the support through binding to the cell binding peptide.
12. The method of claim 11 , wherein the cell binding peptide binds to one or both of urothelial cells and thyroid follicular cells, and wherein the captured cells are for cytological evaluation for one or both of bladder cancer and thyroid cancer.
13. The method of claim 11 , wherein the support is in the form of a slide.
14. The method of claim 11 , wherein the support comprises glass, metal, plastic, synthetic matrix, silica gel, or polymer, derivatives or combinations thereof.
15. The method of claim 11 , wherein the sample comprises blood, plasma, urine, or fine needle aspirate.