Methods and compositions for obtaining useful plant traits
The present disclosure provides methods for obtaining plants that exhibit useful traits by perturbation of organellar function in plants. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants and grafted plants that exhibit, contain, or harbor the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that provide for organellar perturbation are also provided.
1. A method for producing a plant having a useful trait that exhibits nuclear inheritance comprising the steps of:
(a) crossing one or more first plant(s) to one or more second plant(s), or selfing a first plant(s), wherein at least said first plant(s) or a parent plant thereof is or had been subjected to perturbation of organellar function by genetic suppression of an endogenous MSH1 gene with: (i) a transgene, a Viral-Induced Gene Silencing (VIGS) vector, or nucleic acid, wherein said transgene, VIGS vector, or nucleic acid produces or comprises a nucleic acid of at least 18 nucleotides in length that is identical or complementary to the endogenous MSH1 gene; or (ii) a loss of function mutation in the endogenous MSH1 gene; and wherein the first plant or a parent plant thereof does not exhibit any MSH1-dr phenotypes;
(b) screening a population of progeny plants obtained from the cross or self of step (a) for the useful trait, wherein the useful trait is at least one of an increase in biomass or an increase in yield relative to a control plant; and,
(c) selecting one or more progeny plants having the useful trait that exhibits nuclear inheritance and having recovered organellar function, thereby producing a plant exhibiting the useful trait of at least one of an increase in biomass or an increase in yield relative to a control plant, and wherein the useful trait exhibits nuclear inheritance.
2. The method of claim 1 , wherein organellar function has been recovered in any of: (i) the first plant in step (a); (ii) at least a portion of the population of progeny plants of step (b); or (iii) one or more of the selected progeny plants in step (c).
3. The method of claim 1 , wherein the first plant(s) of step (a) exhibit a wild type phenotype or an increase in biomass or an increase in yield relative to a control plant in comparison to a control plant.
4. The method of claim 1 , further comprising the step of producing seed or a progeny plant from the progeny plant selected in step (c), wherein the produced seed or produced progeny plant carry the useful trait, and wherein the useful trait exhibits nuclear inheritance.
5. The method of claim 1 , wherein the plant is a corn, soybean, cotton, canola, wheat, rice, tomato, tobacco, millet, potato, sugarbeet, cassava, alfalfa, barley, oat, sugarcane, sunflower, strawberry, or sorghum plant.
6. The method of claim 1 , wherein the plant is a tomato or sorghum plant.
7. The method of claim 1 , further comprising the step of producing a seed lot from the one or more progeny plants selected in step (c) or from one or more progeny plants obtained therefrom.