Strains and methods for energy partitioning in ruminants
Described are strains including Enterococcus faecium strain 8G-1 (NRRL B-50173), Enterococcus faecium strain 8G-73 (NRRL B-50172), Bacillus pumilus strain 8G-134 (NRRL B-50174) and strains having all of the identifying characteristics of each of these strains. One or more of the strains can be used to reduce negative energy balance in a ruminant. They can also be used to improve other measures of ruminant health and/or performance. Methods of using the strains, alone and in combination, are described. Methods of making the strains are also provided.
1. A method for reducing negative energy balance in a dairy cow comprising:
(a) identifying a dairy cow at risk for negative energy balance; and
(b) administering to the dairy cow identified in step (a) an effective amount in the range of 5×10 8 CFU/animal/day to about 5×10 10 CFU/animal/day of live Bacillus pumilus strain 8G-134 (NRRL B-50174) or a live strain having all of the identifying characteristics of Bacillus pumilus strain 8G-134 (NRRL B-50174) to reduce negative energy balance.
2. The method of claim 1 , wherein the dairy cow is a postpartum dairy cow.
3. The method of claim 1 , wherein the dairy cow is a peripartum dairy cow.
4. The method of claim 1 , further wherein administering to the dairy cow identified in step (a) an effective amount in the range of 5×10 8 CFU/animal/day to about 5×10 10 CFU/animal/day of live Bacillus pumilus strain 8G-134 (NRRL B-50174) or a live strain having all of the identifying characteristics of Bacillus pumilus strain 8G-134 (NRRL B-50174) decreases levels of nonesterified fatty acids by at least 1% or beta-hydroxybutyrate by at least 1% in the blood of the multiparous dairy cow to which is administered the effective amount of live Bacillus pumilus strain 8G-134 (NRRL B-50174) or a live strain having all of the identifying characteristics of Bacillus pumilus strain 8G-134 (NRRL B-50174) when compared to the levels of nonesterified fatty acids or beta-hydroxybutyrate in the blood of a multiparous dairy cow not administered the effective amount of live Bacillus pumilus strain 8G-134 (NRRL B-50174) or a live strain having all of the identifying characteristics of Bacillus pumilus strain 8G-134 (NRRL B-50174).
5. The method of claim 1 , further wherein administering to the dairy cow identified in step (a) an effective amount in the range of 5×10 8 CFU/animal/day to about 5×10 10 CFU/animal/day of live Bacillus pumilus strain 8G-134 (NRRL B-50174) or a live strain having all of the identifying characteristics of Bacillus pumilus strain 8G-134 (NRRL B-50174) increases milk yield in the dairy cow by greater than 0.1% as compared to milk yield of a dairy cow not administered an effective amount of Bacillus pumilus strain 8G-134 (NRRL B-50174) or a strain having all of the identifying characteristics of Bacillus pumilus strain 8G-134 (NRRL B-50174).