Cell counting and sample chamber and methods of fabrication
The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological sample. More particularly, the invention relates to a unique cell counting chamber, e.g., a thin gap fluidic cell chamber for both bright field and fluorescent imaging of bacteria or parasites, and methods for making the same.
1. A method for fabricating a sample chamber, the method comprising:
providing an optically clear backing layer;
placing uniformly an irradiation- or thermally-curable layer on the backing layer;
imprinting a preformed structure mold on to the irradiation- or thermally-curable layer, wherein the preformed structured mold protruding into the irradiation- or thermal-curable layer;
curing the irradiation- or thermally-curable layer by irradiation or thermal treatment;
removing the preformed structure mold, thereby forming an imprinted structure in the irradiation- or thermal-cured layer reflecting the preformed structure of the mold; and
placing an optically clear top film having an optically clear adhesive layer on to the imprinted structure of the irradiation- or thermally-cured layer, thereby forming a chamber having the imprinted structure.
2. The method of claim 1 , further comprising:
forming one or more access port on the chamber allowing introduction and removal of a sample.
3. The method of claim 1 , wherein the optically clear backing layer is made of a material selected from the group consisting of polyester, polycarbonate, PMMA, polystyrene and cyclic olefin copolymer.
4. The method of claim 1 , wherein the irradiation- or thermally-curable layer is made of an irradiation-curable material.
5. The method of claim 1 , wherein the irradiation- or thermally-curable layer is made of an UV-curable material selected from the group consisting of UV-cured acrylate, urethane and epoxy.
6. The method of claim 1 , wherein the irradiation- or thermally-curable layer is made of a thermally-curable material.
7. The method of claim 1 , wherein the optically clear top film is made of a material selected from the group consisting of polyester, polycarbonate, PMMA, polystyrene and cyclic olefin copolymer.
8. The method of claim 1 , wherein the preformed structure mold is formed from aluminum mold.
9. A method for manufacturing a sample chamber suitable for holding a biological sample, the method comprising:
imprinting a preformed structure mold on to an irradiation- or thermal-curable layer, the preformed structured mold protruding into the irradiation- or thermal-curable layer;
curing the irradiation- or thermal-curable layer to form an optically clear layer thereby forming a chamber having the imprinted structure;
removing the preformed structure mold;
binding an optically clear top film to the imprinted structure of the irradiation- or thermal-cured layer, thereby forming a sample chamber; and
forming one or more access port on the chamber allowing introduction and removal of a sample.
10. The method of claim 9 , wherein the irradiation- or thermal-curable layer is an irradiation-curable layer.
11. The method of claim 10 , wherein the irradiation-curable layer is a UV-curable layer.
12. The method of claim 11 , wherein the UV-curable layer is selected from the group consisting of UV-cured acrylate, urethane and epoxy.
13. The method of claim 9 , wherein the optically clear top film is bound to the imprinted structure via an optically clear adhesive layer.
14. The method of claim 9 , wherein the optically clear adhesive layer is selected from the group consisting of polyester, polycarbonate, PMMA, polystyrene and cyclic olefin copolymer.