Genetic test and genetic basis for SCID in pigs
The genetic basis for severe combined immunodeficiency disease (SCID) in pigs is described. In addition, tests for detecting pigs that are carriers for SCID or pigs with SCID are also described. Further, methods for producing pigs or herds of pigs with SCID are also described. Further, methods and compositions for treating, ameilioraing, inhibiting or correcting SCID are provided.
1. A transgenic porcine subject whose genome comprises a transgene comprising the nucleotide sequence of SEQ ID NO: 7 or 9, wherein one allele of a genomic Artemis gene is functionally disrupted by said transgene, wherein said disruption comprises a single nucleotide polymorphism selected from the group consisting of a G to A substitution as set forth at position 615 of SEQ ID NO:7 and a G to A substitution as set forth at position 116 of SEQ ID NO:9, wherein said transgenic porcine subject exhibits severe combined immunodeficiency disease (SCID) relative to a wild type porcine subject, said SCID characterized by decreased numbers of T and B lymphocytes, circulating antibody, and significantly abnormal thymus and lymph nodes.
2. A porcine semen comprising isolated sperm cells from the transgenic porcine subject of claim 1 , wherein the genome of the isolated sperm cells comprises a transgene comprising the nucleotide sequence of SEQ ID NO: 7 or 9, wherein one allele of a genomic Artemis gene is functionally disrupted by said transgene, wherein said disruption comprises a single nucleotide polymorphism selected from the group consisting of a G to A substitution as set forth at position 615 of SEQ ID NO:7 and a G to A substitution as set forth at position 116 of SEQ ID NO:9, wherein said transgenic porcine subject exhibits severe combined immunodeficiency disease (SCID) relative to a wild type porcine subject, said SCID characterized by decreased numbers of T and B lymphocytes, circulating antibody, and significantly abnormal thymus and lymph nodes.
3. A transgenic porcine embryo obtained by fertilizing an oocyte with an isolated sperm cell from the transgenic porcine subject of claim 1 , wherein the genome of the isolated sperm cell comprises a transgene comprising the nucleotide sequence of SEQ ID NO: 7 or 9, wherein one allele of a genomic Artemis gene is functionally disrupted by said transgene, wherein said disruption comprises a single nucleotide polymorphism selected from the group consisting of a G to A substitution as set forth at position 615 of SEQ ID NO:7 and a G to A substitution as set forth at position 116 of SEQ ID NO:9, wherein said transgenic porcine subject exhibits severe combined immunodeficiency disease (SCID) relative to a wild type porcine subject, said SCID characterized by decreased numbers of T and B lymphocytes, circulating antibody, and significantly abnormal thymus and lymph nodes.