Methods for obtaining corn oil from milled corn germ
Methods for obtaining corn oil from milled corn germ (e.g., dry milled corn germ), involving adding water, at least one acidic cellulase, at least one acidic protease, and at least one phytase to milled corn germ to obtain corn oil.
1. A method for obtaining corn oil from milled corn germ, comprising adding water, at least one acidic cellulase, at least one acidic protease, and at least one phytase to said milled corn germ to obtain corn oil; wherein the only enzymes utilized in said method are at least one acidic cellulase, at least one acidic protease and at least one phytase.
2. The method according to claim 1 , wherein said method is conducted for about 1 to about 40 hours.
3. The method according to claim 1 , wherein said method is conducted at about 25° to about 70° C.
4. The method according to claim 1 , wherein said method is conducted at about 35° to about 60° C.
5. The method according to claim 1 , wherein said method is conducted at about 40° to about 55° C.
6. The method according to claim 1 , wherein said method is conducted at about 50° C.
7. The method according to claim 1 , wherein said method is conducted at a pH of about 3 to about 6.
8. The method according to claim 1 , wherein said method is conducted at a pH of about 3.5 to about 5.5.
9. The method according to claim 1 , wherein said method is conducted at a pH of about 4 to about 5.
10. The method according to claim 1 , wherein said method utilizes about 1 to about 5 kg acidic cellulase per Metric Ton (MT) milled corn germ and about 1 to about 5 kg acidic protease per MT milled corn germ and about 0.05 to about 5 kg phytase per MT milled corn germ.
11. The method according to claim 1 , wherein said milled corn germ is selected from the group consisting of dry milled corn germ, wet milled corn germ, and mixtures thereof.
12. The method according to claim 1 , wherein said milled corn germ is milled corn germ.
13. The method according to claim 1 , wherein the yield of corn oil is about 15 to about 70%.
14. The method according to claim 1 , wherein the yield of corn oil is about 30 to about 70%.
15. The method according to claim 1 , wherein the yield of corn oil is about 40 to about 70%.
16. The method according to claim 1 , wherein the yield of corn oil is about 60 to about 70%.
17. The method according to claim 1 , said method consisting essentially of adding water, at least one acidic cellulase, at least one acidic protease, and at least one phytase to said milled corn germ to obtain corn oil.
18. The method according to claim 1 , said method consisting of adding water, at least one acidic cellulase, at least one acidic protease, and at least one phytase to said milled corn germ to obtain corn oil.