System and method for measuring fluorescence of a sample
View Patent ↗The present disclosure provides a system and a method for measuring fluorescence of a sample. The sample may be a polymerase-chain-reaction (PCR) array, a loop-mediated-isothermal amplification array, etc. LEDs are used to excite the sample, and a photodiode is used to collect the sample's fluorescence. An electronic offset signal is used to reduce the effects of background fluorescence and the noises from the measurement system. An integrator integrates the difference between the output of the photodiode and the electronic offset signal over a given period of time. The resulting integral is then converted into digital domain for further processing and storage.
1. A method for measuring fluorescence of a sample, comprising
providing an excitation source configured to irradiate the sample with electromagnetic waves that induce the sample's fluorescence emissions;
providing a detector comprising an integrator circuit and configured to detect fluorescence emitted from the sample;
irradiating, with the excitation source, the sample with electromagnetic waves of wavelengths that that induce the sample's fluorescence emissions;
generating, by the detector, a detection output signal that is proportional to intensities of fluorescence emitted from the sample;
generating, by the detector, a difference between the detection output signal and an offset signal; and
generating, by the integrator circuit, a measurement output signal by integrating the difference over a period of time,
wherein
the offset signal is adjusted to reduce effects of background fluorescence and electronic noises on the measurement output signal.
2. The method of claim 1 ,
wherein the irradiation is performed using a light-emitting diode.
3. The method of claim 1 , further comprising maintaining the sample at a given temperature.
4. The method of claim 1 , further comprising maintaining the sample at a given temperature according to a proportional integral derivative (PID) control algorithm.
5. The method of claim 1 , wherein the offset signal is a constant.
6. The method of claim 1 , wherein the offset signal is a constant such that
the measurement output signal is zero or close to zero when no electromagnetic wave from the excitation source is irradiated on the sample.
7. The method of claim 1 , wherein the offset signal is a constant such that
the measurement output signal is zero or close to zero before any reaction takes place in the sample.
8. The method of claim 1 , wherein the generation of the detection output signal is carried out using a photodiode, a charge-coupled device, or a photomultiplier tube.