Polypeptide hydrogels and uses related thereto
View Patent ↗This disclosure relates to self-assembling peptides that form hydrogels comprising peptide sequences for the activation of signaling pathways. In certain embodiments, the disclosure relates to compositions comprising a recombinant polypeptide comprising a cell signaling sequence, e.g., JAG-1 sequence, fused to a hydrogel polypeptide sequence. In certain embodiments, the disclosure relates to methods of cell culture on three dimensional scaffolds/hydrogels composed of self-assembling peptides disclosed herein.
1. A hydrogel comprising a recombinant polypeptide in combination with a hydrogel polypeptide,
wherein the recombinant polypeptide comprises a cell signaling sequence, wherein the cells signal sequence comprises SEQ ID NO: 1, fused to a hydrogel polypeptide sequence,
wherein the hydrogel polypeptide and the recombinant polypeptide are in a ratio of between 1:5 to 1:15 by weight, and
wherein the hydrogel polypeptide and the recombinant peptide are 1.5% to 2.5% by weight to volume of the hydrogel.
2. The hydrogel of claim 1 comprising a saccharide at a concentration of between 100 nm to 500 mM.
3. The hydrogel of claim 1 comprising a buffer creating a pH of between 7.0 to 7.7.
4. The hydrogel of claim 1 , wherein the hydrogel further comprising a cardiac progenitor cell.