Apparatus, system, and method for collecting a target material
This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a plurality of processing vessels and a collector. The collector funnels portions of the target material from the suspension through a cannula and into the processing vessels. Sequential density fractionation is the division of a sample into fractions or of a fraction of a sample into sub-fractions by a step-wise or sequential process, such that each step or sequence results in the collection or separation of a different fraction or sub-fraction from the preceding and successive steps or sequences. In other words, sequential density fractionation provides individual sub-populations of a population or individual sub-sub-populations of a sub-population of a population through a series of steps.
1. A system comprising:
a primary vessel comprising an open end and a biological suspension comprising a target material;
a processing vessel comprising
a first end comprising a plug,
an inner cavity, and
a displacement fluid having a density greater than a density of the target material, wherein the displacement fluid is located within the inner cavity; and
a collector comprising an opening in a bottom end and a cavity in a top end connected by a cannula,
wherein the bottom end of the collector is located within the open end of the primary vessel and the top end extends upwardly out of the open end of the primary vessel,
wherein at least a portion of the processing vessel is located within the cavity of the collector, and
wherein the cannula extends through the plug of the processing vessel and accesses the inner cavity of the processing vessel.
2. The system of claim 1 , the primary vessel further comprising a clearing fluid having a density greater than the density of the displacement fluid.
3. The system of claim 1 , wherein the displacement fluid is selected from the group consisting of: a solution of colloidal silica particles coated with polyvinylpyrrolidone, a polysaccharide solution, iodixanol, an organic solvent, a liquid wax, an oil, a gas, olive oil, mineral oil, silicone oil, immersion oil, mineral oil, paraffin oil, silicon oil, fluorosilicone, perfluorodecalin, perfluoroperhydrophenanthrene, perfluorooctylbromide, organic solvents, 1,4-Dioxane, acetonitrile, ethyl acetate, tert-butanol, cyclohexanone, methylene chloride, tert-Amyl alcohol, tert-Butyl methyl ether, butyl acetate, hexanol, nitrobenzene, toluene, octanol, octane, propylene carbonate, tetramethylene sulfones, ionic liquids, a polymer-based solution, a surfactant, a perfluoroketone, perfluorocyclopentanone, perfluorocyclohexanone, a fluorinated ketone, a hydrofluoroether, a hydrofluorocarbon, a perfluorocarbon, a perfluoropolyether, silicon, a silicon-based liquid, phenylmethyl siloxane, and combinations thereof.
4. The system of claim 1 , wherein the plug is resealable.
5. The system of claim 1 , further comprising:
a float located at a longitudinal position within the primary vessel.
6. The system of claim 5 , further comprising a sealing ring located circumferentially around the primary vessel at the same longitudinal position as at least a portion of the float within the primary vessel.
7. The system of claim 1 , wherein the cannula is a tube, a needle, or a non-coring needle.
8. The system of claim 1 , wherein the cannula has a flat tip, a beveled tip, a sharpened tip, or a tapered tip.
9. The system of claim 1 , further comprising a fluid-tight seal between the bottom end of the collector and an inner wall of the primary vessel.
10. The system of claim 9 , wherein the fluid-tight seal is formed by an interference fit, a grease, vacuum grease, an adhesive, an epoxy, thermal bonding, welding, ultrasonic welding, a ring, a clamp, or an insert that fits between the bottom end of the collector and the inner wall of the primary vessel.
11. The system of claim 1 , the collector further comprising a shoulder extending circumferentially around a main body of the collector.
12. The system of claim 11 , further comprising a lock ring placed over the shoulder of the collector and the open end of the primary vessel.
13. The system of claim 1 , the processing vessel further comprising a processing solution to effect a transformation on the target material.
14. The system of claim 13 , wherein the processing solution is a preservative, a cell adhesion solution, or a dye.