Microfluidic device for cell capture and isolation

1. A device for capturing a biological cell from a sample, the device comprising:

a capture bed comprising:

a wave-herringbone surface pattern having a ratio of amplitude-to-wavelength that is 1:2; and

a plurality of nanostructures;

wherein the wave-herringbone pattern and the plurality of nanostructures are integrated on a single surface; and

wherein the nanostructures are equidistantly spaced in the capture bed.

2. The device according to claim 1 , wherein the nanostructures are selected from the group consisting of nanopillars; nanospheres; and a combination thereof.

3. The device according to claim 1 , wherein the nanostructures each have an average diameter of from 100 nm to 1500 nm.

4. The device according to claim 1 , wherein the equidistant spacing between nanostructures be selected from a distance ranging from 100 nm to 250 nm.

5. The device according to claim 1 , wherein the wave-herringbone surface pattern comprises sinusoidal-like waves extending in two directions.

6. The device according to claim 1 , wherein the wave-herringbone surface pattern is configured to form channels in the capture bed.

7. The device according to claim 6 , wherein the channels have a height of from 20 μm to 100 μm.

8. The device according to claim 1 , further comprising:

an inlet; and

an outlet;

wherein the outlet is positioned at a distance from the inlet; and wherein the sample flows from the inlet to the outlet.

9. The device according to claim 8 , wherein the sample flows from the inlet to the outlet at a rate sufficient to capture a biological cell from the sample.

10. The device according to claim 1 , wherein the capture bed further comprises a capturing agent.

11. The device according to claim 10 , wherein the capturing agent is selected from the group consisting of: an antibody; and an aptamer.

12. A kit for isolating a target cell from a sample comprising the device according to claim 1 ; and instructions for using said device.