METHOD FOR DETECTING NUCLEIC ACID
A microarray is disclosed which contains a plurality of gels having different gel concentrations which are carried in respective hollow portions of the microarray, and in which a probe is immobilized in the gels. A method for producing such a microarray is also disclosed.
1 . A microarray, comprising a plurality of gels having different gel concentrations which are carried in respective hollow portions of the microarray, wherein a probe is immobilized in the gels.
2 . The microarray of claim 1 , wherein a ratio (V/S) of a volume of the gels (V(μm 3 )) to a contact surface area of the gels on which the probe is immobilized and a reaction solution (S(μm 2 )) is 50 or more.
3 . The microarray of claim 1 , wherein the gels on which the probe is immobilized are held in a well or through-hole in a substrate.
4 . The microarray of claim 1 , wherein the gels on which the probe is immobilized comprise a substituted (meth)acrylamide derivative, an agarose derivative, or both.
5 . The microarray of claim 1 , wherein gel concentrations of the gels on which the probe is immobilized are more than 2% by mass and less than 5% by mass.
6 . A method for producing the microarray of claim 1 , the method comprising:
three-dimensionally arranging a plurality of hollow fibers so that fiber axial directions of the hollow fibers become the same, and wherein the arrangement is fixed with a resin to produce a hollow fiber bundle;
introducing a plurality of gel precursor solutions having different monomer concentrations comprising the probe into respective hollow portions of the hollow fibers of the hollow fiber bundle;
reacting the gel precursor solutions introduced into the hollow portions to obtain gel-like products comprising the probe in the hollow portions of the hollow fibers; and
slicing the hollow fiber bundle in a direction crossing a longitudinal direction of the fibers into thin sections.