Extract of taiwanese propolis for treating ocular diseases
View Patent ↗The present invention provides a method for treating an ocular disease in a subject in need thereof, comprising administering an effective amount of an extract of Taiwanese propolis (TP) or an active component thereof to the subject. Also provided is a pharmaceutical composition for treating an ocular disease, which comprises an effective amount of an extract of TP or an active component thereof, and a pharmaceutically acceptable carrier.
1. A method for treating an ocular disease in a subject in need thereof, comprising
administering to the subject an extract of Taiwanese propolis (TPE) in an amount effective to enhance the viability of RPE cells suffering from oxidative stress and/or hypoxia-induced damages, and at the same time having little or no effect on the viability of human umbilical vein endothelial cells (HUVECs),
wherein the TPE is prepared from Taiwanese propolis by a method comprising the following steps:
(i) extracting Taiwanese propolis with 95% ethanol for about 48 hours at 45° C. to obtain a first extract;
(ii) collecting a soluble fraction of the first extract containing propolins; and
(iii) concentrating the soluble fraction by about 4 times to obtain the TPE,
whereby each gram of TPE contains no less than 100 mg of propolin C, 80 mg of propolin D, 30 mg of propolin F and 50 mg of propolin G, and each gram of TPE contains no less than 350 mg of total propolins.
2. The method of claim 1 , wherein the ocular disease is selected from the group consisting of glaucoma, age-related macular degeneration (AMD), ischemic retinopathy, optic neuropathy, diabetic retinopathy, diabetic macular edema, uveitis, and senile cataract.
3. The method of claim 2 , wherein the ocular disease is AMD.
4. The method of claim 3 , wherein the AMD is dry AMD.
5. The method of claim 1 , wherein the TPE is administered through oral administration, parenteral injection, intravitreal injection, or skin patch.
6. The method of claim 1 , wherein the TPE is formulated into eye ointment, eye gel, eye cream, or eye drops.
7. The method of claim 1 , wherein the ocular disease is not an eye cancer.
8. The method of claim 1 , wherein the Taiwanese propolis is Taiwanese green propolis.
9. The method of claim 1 , wherein the TPE is administered orally to the subject 1-3 times a day, with each oral administration of having an amount of the TPE of 10-200 mg.
10. The method of claim 1 , wherein the TPE is administered ophthalmologically to the subject 1-10 times daily, with each ophthalmological administration using a solution of 0.01-10% TPE.