Key gene regulating plant cell wall recalcitrance
This disclosure provides plants having desirable levels of lignin synthesis, sugar release, S/G ratio, and resistance to stress and pathogens; methods of selecting plants with such desirable levels of lignin synthesis, sugar release, S/G ratio, and resistance to stress and pathogens; methods of genetically modifying plants to modulate lignin synthesis, sugar release, S/G ratio, and resistance to stress and pathogens; and uses of such plants. The inventors have determined that the expression and/or activity of POPTR_0014s08530, a gene encoding an Angustifolia /CtBP transcription factor, modulates lignin synthesis, sugar release, S/G ratio, and resistance to stress and pathogens in plants. Plants with lignin synthesis, sugar release, S/G ratio, and resistance to stress and pathogens, based on modulation of the expression or activity of the POPTR_0014s08530 gene, have divergent uses including pulp and paper production, and ethanol/biofuel production.
1. A method comprising:
a. identifying a plant comprising an allelic variant of the POPTR 0014s08530 gene wherein said allelic variant encodes an increased number of glutamines relative to the sequence of SEQ ID NO:2, wherein said identifying comprises obtaining nucleic acids from a plant and detecting the presence of said allelic variant; and
b. producing biofuel or cellulose-based products from said plant.
2. A method comprising:
a. identifying a plant comprising an allelic variant of the POPTR_0014s08530 gene wherein said allelic variant encodes a decreased number of glutamines relative to the sequence of SEQ ID NO:2, wherein said identifying comprises obtaining nucleic acids from a plant and detecting the presence of said allelic variant; and
b. producing lignin-based products from said plant.
3. The method of claim 1 , wherein the allelic variant present in said plant encodes a polypeptide with at least 85% sequence identity to SEQ ID NO: 2.
4. The method of claim 1 , wherein the allelic variant encodes SEQ ID NO: 4.
5. The method of claim 3 , wherein the allelic variant encodes a polypeptide with at least 95% sequence identity to SEQ ID NO: 2.
6. The method of claim 1 , wherein the detection in step (a) is by polymerase chain reaction or nucleic acid hybridization.
7. The method of claim 2 , wherein the lignin-related product is a carbon fiber.
8. The method of claim 1 , wherein the cellulose-related products are selected from the group consisting of cellulose, paper and pulp.
9. The method of claim 2 , wherein the allelic variant present in said plant encodes a polypeptide with at least 85% sequence identity to SEQ ID NO:2.
10. The method of claim 2 , wherein the allelic variant encodes a polypeptide with at least 95% sequence identity to SEQ ID NO:2.
11. The method of claim 2 , wherein the detection in step (a) is by polymerase chain reaction or nucleic acid hybridization.
12. The method of claim 5 , wherein the allelic variant encodes a polypeptide with at least 98% sequence identity to SEQ ID NO: 2.
13. The method of claim 2 , wherein the allelic variant encodes a polypeptide with at least 98% sequence identity to SEQ ID NO: 2.
14. The method of claim 10 , wherein the allelic variant differs from SEQ ID NO: 2 by having 11 glutamines.