Cryosolutions and uses thereof
Compositions for maintaining cellular integrity especially in cases of cryopreservation and multiple freeze/thawing cycles are provided. In addition, the compositions can be used in various media for a variety of applications.
1. A composition for cryopreservation consisting of: polyethylene glycol (PEG), a saccharide, and an organic amphoteric agent, wherein the polyethylene glycol is present in the composition from 0.001% to 20% v/v, the saccharide is present in the composition from 0.1 μg/ml to 1 mg/ml and the organic amphoteric agent is present in the composition from 0.1% to 20% v/v, wherein:
said organic amphoteric agent is ectoin and/or hydroxyectoin;
said saccharide is trehalose and/or dextran-40;
said composition being added to a biological medium.
2. The composition of claim 1 , wherein the composition consists of from 0.1 μg/ml to 0.9 mg/ml of trehalose and/or dextran-40, from 0.1% to 20% v/v ectoin and/or hydroxyectoin and from 0.001% to 20% v/v of PEG.
3. The composition of claim 1 , wherein the composition comprises from 0.1 μg/ml to 800 μg/ml of trehalose.
4. The composition of claim 1 , wherein the composition comprises from 0.1 μg/ml to 700 μg/ml of trehalose.
5. The composition of claim 1 , wherein the composition comprises from 0.1 μg/ml to 500 μg/ml of trehalose.
6. The composition of claim 1 , wherein the composition comprises from 0.1 μg/ml to 250 μg/ml of trehalose.
7. The composition of claim 1 , wherein the composition comprises from 0.1 μg/ml to 100 μg/ml of trehalose.
8. The composition of claim 1 , wherein the composition comprises from 0.1 μg/ml to 50 μg/ml of trehalose.