Bone morphogenic protein binding peptide
A cyclized peptide designated BMP Binding Peptide (BBP) is a synthetic peptide that avidly binds rhBMP-2, as do endogenous forms of BBP, and sequence conservation between species results in a variety of useful BBP compositions. BBP increases the over-all osteogenic activity of rhBMP-2, increases the rate at which rhBMP-2 induces bone formation, and BBP induces calcification alone. Compositions and substrates including BBP, and methods of using BBP are useful in therapeutic, diagnostic and clinical applications requiring calcification and osteogenesis.
1. An article of manufacture comprising an implant, wherein said implant has a surface, and wherein said surface includes a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification in vertebrates.
2. The article of manufacture of claim 1 wherein the implant further includes one or more of TGF-beta, BMP-2, BMP-4, BMP-7 or demineralized bone matrix.
3. The article of manufacture of claim 1 wherein the implant further includes mesenchymal stem cells.
4. The article of manufacture of claim 3 wherein the mesenchymal stem cells secrete one or more growth factors.
5. The article of manufacture of claim 4 wherein the growth factor is a TGF-beta family member.
6. The article of manufacture of claim 1 wherein the implant further includes chondrogenic or osteogenic precursor cells.
7. The article of manufacture of claim 6 wherein the chondrogenic or osteogenic precursor cells secrete one or more growth factors.
8. The article of manufacture of claim 7 wherein the growth factor is a TGF-beta family member.
9. The article of manufacture of claim 1 wherein said implant is formed into the shape of a pin, screw, plate, or prosthetic joint.
10. The article of manufacture of claim 1 wherein said peptide is immobilized on the surface of said implant.
11. An article of manufacture comprising a solid support, wherein said solid support includes a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification in vertebrates.
12. The article of manufacture of claim 11 wherein said solid support has a surface, and wherein said peptide is immobilized on said surface.
13. The article of manufacture of claim 11 wherein the solid support further includes one or more of TGF-beta, BMP-2, BMP-4, BMP-7 or demineralized bone matrix.
14. The article of manufacture of claim 11 wherein the solid support further includes mesenchymal stem cells.
15. The article of manufacture of claim 14 wherein the mesenchymal stem cells secrete one or more growth factors.
16. The article of manufacture of claim 15 wherein the growth factor is a TGF-beta family member.
17. The article of manufacture of claim 11 wherein the solid support further includes chondrogenic or osteogenic precursor cells.
18. The article of manufacture of claim 17 wherein the chondrogenic or osteogenic precursor cells secrete one or more growth factors.
19. The article of manufacture of claim 18 wherein the growth factor is a TGF-beta family member.
20. A method of enhancing the degree or rate of osteogenesis or calcification in vertebrate tissue comprising applying to the tissue an implant, wherein said implant has a surface, and wherein said surface includes a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification in vertebrates.
21. The method of claim 20 wherein implant is applied to the tissue at a location proximate to the desired location of osteogenesis or calcification.
22. The method of claim 20 wherein the implant further includes mesenchymal stem cells.
23. The method of claim 22 wherein the mesenchymal stem cells secrete one or more growth factors.
24. The method of claim 23 wherein the growth factor is a TGF-beta family member.
25. The method of claim 20 wherein the implant further includes chondrogenic or osteogenic precursor cells.
26. The method of claim 25 wherein the chondrogenic or osteogenic precursor cells secrete one or more growth factors.
27. The method of claim 26 wherein the growth factor is a TGF-beta family member.