Method of synthesis of gene library using codon randomization and mutagenesis
Proposed is a method of easily finding an error during analysis of various library sequences of nucleic acid base sequences after synthesizing a gene library using a combination of three nucleic acid base sequences (codon) translated into the same protein. This shows that it is possible to create a gene library having the same protein sequence but different nucleic acid base sequences. The present disclosure provides a novel experimental method capable of measuring a correlation between gene expression according to change in a codon by changing the usage of a codon optimized to express a specific gene in vivo.
1. A method of synthesizing a second gene library, the method comprising: providing a first gene library comprising randomized codons encoding to a specific protein sequence but having different nucleic acid base sequences; fragmenting the first gene library into nucleic acid fragments; confirming base sequences of the nucleic acid fragments; and reassembling the base sequence-confirmed nucleic acid fragments into gene sequences before fragmentation using the codon-randomized base sequences.
2. The method according to claim 1 , wherein, in the providing, the randomized codons are prepared by combining different nucleic acid base sequences coding the same protein by inserting degenerate codons into gene sequences coding a specific protein sequence.
3. The method according to claim 1 , wherein, in the providing, both ends of gene sequences of randomized codons in the first gene library are provided with at least one selected from the group consisting of barcode tag sequences, restriction enzyme recognition sequences and flanking sequences.
4. The method according to claim 1 , further comprising controlling the number of codon-randomized gene sequences in the first gene library to a verifiable number through next generation sequencing after the providing.
5. The method according to claim 1 , wherein, in the confirming, the sequence confirmation is performed using a next generation sequencing.
6. The method according to claim 1 , wherein the reassembling comprises: finding mapping locations of the base sequence-confirmed nucleic acid fragments using the codon-randomized base sequences; and connecting the nucleic acid fragments having overlapping base sequences with respect to the mapped locations.
7. The method according to claim 6 , wherein, in the connecting, lengths of the overlapping sequences are 20 to 80 bp.
8. A gene library prepared by the method of claim 1 , wherein the gene library is the gene library without sequencing errors produced by using an assembly depth such that the gene sequence has a recovery rate of 99.9% or more based on the gene sequence before the fragmentation.
9. A method of synthesizing a library of mutated genes, the method comprising: providing a gene library comprising randomized codons encoding to a specific protein sequence but having different nucleic acid base sequences; inducing mutagenesis of the gene library; fragmenting the mutated gene library into nucleic acid fragments; confirming base sequences of the nucleic acid fragments; and reassembling the base sequence-confirmed nucleic acid fragments into gene sequences before fragmentation using codon-randomized base sequences.
10. The method according to claim 9 , wherein, in the inducing, the mutagenesis is random mutagenesis.
11. The method according to claim 9 , wherein, in the inducing, the mutagenesis is performed by mutagenesis amplification.
12. The method according to claim 11 , wherein the mutagenesis amplification is error prone PCR.
13. The method according to claim 9 , wherein the library of the mutated genes is a cluster of different base sequences encoding a protein having a mutated amino acid in a specific location.
14. The method according to claim 9 , wherein, the library of the mutated genes is a cluster of different base sequences encoding a protein having random mutations in an entire amino acid region.