Patent
The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus , and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.
1. A method for thermocyclic amplification of nucleic acid comprising:
(a) contacting a nucleic acid with a thermostable polypeptide having any one of SEQ ID NO: 13-24 under conditions suitable for amplification of said nucleic acid; and
(b) amplifying the nucleic acid.
2. The method of claim 1 wherein the thermocyclic amplification of the nucleic acid includes cycles of denaturation, primer annealing and primer extension.
3. The method of claim 1 wherein the thermocyclic amplification of the nucleic acid is performed by Strand Displacement Amplification.
4. The method of claim 1 wherein thermocyclic amplification of the nucleic acid is performed by Polymerase Chain Reaction.