NNA-DNA adducts as biomarkers for detecting exposure to thirdhand smoke
View Patent ↗NNA-derived specific adducts represent an integrated biomarker of exposure to thirdhand smoke (THS) as NNA is unique to THS. The NNA-dG covalent binding adduct could serve as such a biomarker, and play a role in identifying individuals exposed to THS, thus providing critical information for early detection and prevention.
1. A method for detection of prior thirdhand smoke (THS) exposure in a sample, comprising the steps of
a. obtaining a biological or non-biological sample, wherein said sample is not a biological sample obtained from a firsthand smoker or non-smokers exposed to secondhand smoke (SHS);
b. providing a detector which detects the presence or level of a 2-deoxyguanosine (dG) adduct, and 1-(N-methyl-N-nitrosamino)-1-(3-pyridinyl)-4-butanal (NNA) in a sample; and
c. detecting the presence of said dG adduct, and NNA by the detector above standard baseline levels;
wherein the dG adduct detected having the structure of:
2. The method of claim 1 , wherein the detection of dG adduct presence or level is detected using mass spectrometry or immunohistochemistry.
3. The method of claim 2 , wherein the detection is using immunohistochemistry.
4. The method of claim 3 , prior to the providing step (a), the method further comprising the step of contacting the sample with an antibody specific for the dG adduct to be detected.
5. The method of claim 1 , wherein the sample is a human tissue or bodily fluid.
6. The method of claim 5 , wherein the sample is a bodily fluid selected from the group consisting of skin, blood, urine, saliva, and fecal sample.
7. The method of claim 6 , wherein the sample is blood.
8. A method for the detection of the presence of DNA damage in a cell as a result of exposure to thirdhand smoke, comprising the steps of:
(a) providing a cell suspected of exposure to thirdhand smoke;
(b) detecting the presence or level of an NNA and a dG adduct of claim 1 in said cell;
(c) determining the level of DNA breaks that the cell has encountered due to thirdhand smoke using NNA and dG adduct-specific probes or compositions to specifically detect NNA and dG adducts as thirdhand smoke biomarkers at the chromosomal location of the DNA break.
9. The method of claim 8 , wherein the dG adduct is 8-Oxo-dG; O 6 -methyl-dG; N 2 -methyl-dG; 5′,3′-Methyl-dG; or 1, N 2 -NNA-dG.
10. The method of claim 8 , wherein the cell is from a human tissue or bodily fluid sample.
11. The method of claim 10 , wherein the cell is from a bodily fluid sample and the bodily fluid is selected from the group consisting of blood, urine, saliva, and feces.
12. The method of claim 11 , wherein the cell is from a blood sample.
13. The method of claim 1 , further comprising step c of initiating an notification signal to indicate the detector has detected the presence and level of a dG adduct or NNA described in step c of claim 1 .