Reagents and methods for breast cancer detection
The present invention provides compositions including reagents for detecting human autoantibodies against at least two proteins selected from the group consisting of ANGTPL4, DKK1, EPHA2, LAMC2, SPON2, SSR2, GAL1, GFRA1, LRRC15, CD147, CD320, CDH3, LRP10, SPINT2, SUSD2, and CST2, and their use in detecting breast cancer or disease recurrence.
1. A composition consisting of between 2 and 25 proteins, or antigenic fragments thereof, wherein the between 2 and 25 proteins or antigenic fragments thereof include at least (i) ANGPTL4 or an ANGPTL4 antigenic fragment, and (ii) DKK1 or a DKK1 antigenic fragment, wherein:
(a) the proteins, or antigenic fragments thereof are detectably labeled; and/or
(b) the proteins, or antigenic fragments thereof are immobilized on a surface, wherein no additional proteins or antigenic fragments thereof are present on the surface.
2. The composition of claim 1 , wherein the between 2 and 25 proteins or antigenic fragments thereof include at least one proteins selected from the group consisting of ANGPTL4, DKK1, EPHA2, GAL1, LAMC2, SPON2, CST2, SPINT2 and SSR2, or antigenic fragments thereof.
3. The composition of claim 1 , wherein the between 2 and 25 proteins or antigenic fragments thereof include at least three proteins selected from the group consisting of EPHA2, LAMC2, SPON2, SSR2, GAL1, GFRA1, LRRC15, CD147, CD320, CDH3, LRP10, SPINT2, SUSD2, and CST2, or antigenic fragments thereof.
4. The composition of claim 1 , wherein the between 2 and 25 proteins or antigenic fragments include one or both of MUC1 protein and GRN protein, or antigenic fragments thereof.
5. The composition of claim 1 , wherein the composition consists of between 2 and 20 proteins, or antigenic fragments thereof.
6. The composition of claim 1 , wherein the composition consists of between 4 and 10 proteins, or antigenic fragments thereof.
7. The composition of claim 1 , wherein the composition consists of between 5 and 10 proteins, or antigenic fragments thereof.
8. The composition of claim 1 , wherein the composition includes proteins, or antigenic fragments thereof selected from one of the following sets:
ANGPTL4, DKK1, GAL1, GFRA1, GRANULIN, LRRC15, and MUC1;
ANGPTL4, DKK1, GAL1, GRANULIN, LRRC15, and MUC1;
ANGPTL4, DKK1, GAL1, and LRRC15;
ANGPTL4, DKK1, GAL1, GFRA1, and LRRC15;
ANGPTL4, DKK1, GAL1, GFRA1, GRANULIN, and LRRC15;
ANGPTL4, DKK1, GAL1, GRANULIN, and LRRC15; and
ANGPTL4, DKK1, GAL1, LRRC15, and MUC1.
9. The composition of claim 1 , wherein the composition includes ANGPTL4, DKK1, GAL1, MUC1, GFRA1, GRN and LRRC15 proteins, or antigenic fragments thereof.
10. The composition of claim 1 , wherein the between 2 and 25 proteins, or antigenic fragments thereof comprise native extracellular domains and/or native secreted proteins or antigenic fragments thereof.
11. The composition of claim 1 , wherein the proteins, or antigenic fragments thereof are detectably labeled.
12. The composition of claim 1 , wherein the proteins, or antigenic fragments thereof are immobilized on a surface.
13. The composition of claim 2 , wherein the between 2 and 25 proteins, or antigenic fragments thereof comprise native extracellular domains and/or native secreted proteins or antigenic fragments thereof.
14. The composition of claim 2 , wherein the proteins, or antigenic fragments thereof are detectably labeled.
15. The composition of claim 2 , wherein the proteins, or antigenic fragments thereof are immobilized on a surface.
16. The composition of claim 8 , wherein the between 2 and 25 proteins, or antigenic fragments thereof comprise native extracellular domains and/or native secreted proteins or antigenic fragments thereof.
17. The composition of claim 8 , wherein the proteins, or antigenic fragments thereof are detectably labeled.
18. The composition of claim 8 , wherein the proteins, or antigenic fragments thereof are immobilized on a surface.
19. The composition of claim 1 , wherein the between 2 and 25 proteins, or antigenic fragments thereof comprise native extracellular domains and/or native secreted proteins or antigenic fragments thereof, and wherein the proteins, or antigenic fragments thereof are immobilized on a surface.
20. The composition of claim 2 , wherein the between 2 and 25 proteins, or antigenic fragments thereof comprise native extracellular domains and/or native secreted proteins or antigenic fragments thereof, and wherein the proteins, or antigenic fragments thereof are immobilized on a surface.
21. The composition of claim 8 , wherein the between 2 and 25 proteins, or antigenic fragments thereof comprise native extracellular domains and/or native secreted proteins or antigenic fragments thereof, and wherein the proteins, or antigenic fragments thereof are immobilized on a surface.