Biological cell separator and disposable kit
A disposable kit for use in directing fluid through a biological cell separator device ( 10 ). The kit generally includes a separator tube ( 22 ), a buffer fluid container ( 34 ), cell sample container ( 32 ), separated cell container ( 60 ), and flushing fluid container ( 62 ), as well as various conduits ( 36, 38, 42, 50, 50 a, 50 b ) for connecting the containers ( 32, 34, 60, 62 ) and separator tube ( 22 ) in fluid communication together. A cell separator system is provided including a separator tube ( 22 ), magnet ( 20 ), pump ( 120 ) and a motorized drive unit ( 96 ). The motorized drive unit ( 96 ) is operatively connected to the magnet ( 20 ) to allow the magnet ( 20 ) to be moved a sufficient distance away from the separator tube ( 22 ) so as to allow cells adhered to the inside surface thereof to be flushed out of the tube ( 22 ).
1. A disposable kit for directing a fluid sample of biological cells through a separator device and collecting separated cells from the sample, the kit comprising:
a sample receiving container including an inlet and an outlet, the inlet coupled with an inlet conduit for receiving a buffer fluid and the outlet coupled with an outlet conduit, the outlet conduit comprising a first section and a second section;
a separator tube including an inlet end and an outlet end, the inlet end coupled with the outlet conduit from the sample receiving container, the separator tube having:
a length defining a separation channel;
a diameter adapted for operable communication with a magnet along the length of the separation channel;
a space for receiving a flow of the buffer fluid and sample, and wherein a length of the second section of the outlet conduit from the sample receiving container is oriented along a path defined by the separator tube and adapted to allow displacement of the magnet from a separation position to an idle position while the flow of fluid is maintained;
a separated cell container having an inlet port and a vacuum port,
a separated cell conduit coupled for fluid communication between the outlet end of the separator tube and the inlet port of the separated cell container,
a flushing fluid container having an inlet port and a vacuum port; and
a flushing conduit coupled for fluid communication between the outlet end of the separator tube and the inlet port of the flushing fluid container.
2. The kit of claim 1 , further comprising:
a buffer fluid container coupled with the inlet conduit to the sample receiving container.
3. The kit of claim 1 , wherein at least a portion of the outlet conduit from the sample receiving container comprises a flexible tubing for receipt within a first pinch valve.
4. The kit of claim 1 , wherein at least a portion of the separated cell conduit comprises a flexible tubing for receipt within a second pinch valve.
5. The kit of claim 1 , wherein at least a portion of the flushing conduit comprises a flexible tubing for receipt within a third pinch valve.
6. The kit of claim 1 , further comprising a venting conduit connected for fluid communication with the flushing fluid container.
7. The kit of claim 1 , further comprising a first pump conduit coupled with the vacuum port of the separated cell container, and a second pump conduit coupled with the vacuum port of the flushing fluid container.