Altered microbiome of chronic pelvic pain
Provided herein are kits, compositions, and methods for diagnosing and treating interstitial cystitis (IC) and/or interstitial cystitis/bladder pain syndrome (IC/BPS) based on finding lower levels of certain bacteria in a subject's stool sample (e.g., O. splanchnicus, F. prausnitzii, C. aerofaciens, E. sinensis, L. longoviformis , and R. intestinalis ). In certain embodiments, then present invention provides probiotic formulations containing live bacteria (e.g., from O. splanchnicus, F. prausnitzii, C. aerofaciens, E. sinensis, L. longoviformis , and R. intestinalis ).
1. A method comprising:
testing, or having tested a stool sample from a subject suspected of suffering from interstitial cystitis (IC), to determine the level of bacteria from at least one bacterial species from a bacterial genus,
wherein said bacterial genus is selected from the group consisting of: Odoribacter, Faecalibacterium, Colinsella, Eggerthella, Lactonifactor , and Roseburia.
2. The method of claim 1 , wherein said at least one species is selected from the group consisting of: Odoribacter splanchnicus, Faecalibacterium prausnitzii, Colinsella aerofaciens, Eggerthella sinensis, Lactonifactor longoviformis , and Roseburia intestinalis.
3. The method of claim 1 , wherein said at least one species is at least two species selected from the group consisting of: Odoribacter splanchnicus, Faecalibacterium prausnitzii, Colinsella aerofaciens, Eggerthella sinensis, Lactonifactor longoviformis , and Roseburia intestinalis.
4. The method of claim 1 , wherein said at least one species is at least five species selected from the group consisting of: Odoribacter splanchnicus, Faecalibacterium prausnitzii, Colinsella aerofaciens, Eggerthella sinensis, Lactonifactor longoviformis , and Roseburia intestinalis.
5. The method of claim 1 , wherein said stool sample is tested with a sequencing assay specific to ribosomal RNA of said at least one species.
6. The method of claim 1 , further comprising comparing the level of bacteria to healthy control population levels.