Signal biomarkers
Diagnostics relating to C-type natriuretic and erythropoietin signal peptides and fragments, and kits, uses and applications therefore.
1. An antibody, or antigen-binding fragment thereof, which specifically binds a peptide of C-type natriuretic signal peptide CNPsp selected from CNPsp(1-13) peptide having an amino acid sequence consisting of SEQ ID NO:5 or CNPsp(14-23) peptide having an amino acid sequence consisting of SEQ ID No:6,
wherein the antibody, or antigen-binding fragment thereof is immobilized on a solid matrix or is labeled.
2. The antibody, or antigen-binding fragment thereof, of claim 1 , wherein the antibody, or antigen-binding fragment thereof, is a polyclonal, monoclonal, chimeric, or humanized antibody or antigen-binding fragment thereof.
3. The antibody, or antigen-binding fragment thereof, of claim 1 wherein the antibody, or antigen-binding fragment thereof, is labelled with a detectable marker.
4. The antibody, or antigen-binding fragment thereof, of claim 1 , wherein the antibody, or antigen-binding fragment thereof, specifically binds CNPsp(1-13) peptide having an amino acid sequence consisting of SEQ ID NO:5.
5. The antibody, or antigen-binding fragment thereof, of claim 1 , wherein the antibody, or antigen-binding fragment thereof, specifically binds CNPsp(14-23) peptide having an amino acid sequence consisting of SEQ ID NO:6.
6. The antibody, or antigen-binding fragment thereof, of claim 1 , wherein the antibody, or antigen-binding fragment thereof, is in a complex bound to a C-type natriuretic signal peptide (CNPsp) to which the antibody, or antigen-binding fragment thereof, specifically binds.
7. The antibody, or antigen-binding fragment thereof, of claim 1 , wherein the antibody, or antigen-binding fragment thereof, is immobilized on a solid matrix.
8. The antibody, or antigen-binding fragment thereof, of claim 6 , wherein the antibody, or antigen-binding fragment thereof, is immobilized on a solid matrix.
9. A composition comprising:
a first anti-C-type natriuretic signal peptide (CNPsp) antibody, or antigen-binding fragment thereof, immobilized on a solid matrix;
a CNPsp or fragment thereof bound to the first anti-CNPsp antibody, or antigen-binding fragment thereof; and
a second anti-CNPsp antibody, or antigen-binding fragment thereof, bound to the CNPsp or fragment thereof, wherein the second anti-CNPsp antibody, or antigen-binding fragment thereof, binds to a site on CNPsp or fragment thereof that is different from the site bound by the first anti-CNPsp antibody, or antigen-binding fragment thereof, wherein
either the first or the second anti-CNPsp antibody, or antigen-binding fragment thereof, is the anti-CNPsp antibody, or antigen-binding fragment thereof, which specifically binds a peptide of CNPsp selected from CNPsp(1-13) peptide having an amino acid sequence consisting of SEQ ID NO:5 or CNPsp(14-23) peptide having an amino acid sequence consisting of SEQ ID NO:6.
10. The composition of claim 9 , wherein the second anti-CNPsp antibody, or antigen-binding fragment thereof, is labeled.
11. The composition of claim 9 , wherein either the first or the second anti-CNPsp antibody, or antigen-binding fragment thereof, binds the CNPsp(1-13) peptide having an amino acid sequence consisting of SEQ ID NO:5.
12. The composition of claim 9 , wherein either the first or the second anti-CNPsp antibody, or antigen-binding fragment thereof, binds the CNPsp(14-12) peptide having an amino acid sequence consisting of SEQ ID NO:6.
13. A kit comprising:
a first anti-C-type natriuretic signal peptide (CNPsp) antibody, or antigen-binding fragment thereof, immobilized on a solid matrix; and
a second anti-CNPsp antibody, or antigen-binding fragment thereof, wherein the second anti-CNPsp antibody, or antigen-binding fragment thereof, binds to a site on CNPsp or fragment thereof that is different from the site bound by the first anti-CNPsp antibody, wherein
either the first or the second anti-CNPsp antibody, or fragment thereof, is the anti-CNPsp antibody, or antigen-binding fragment thereof, which specifically binds a peptide of CNPsp selected from CNPsp(1-13) peptide having an amino acid sequence consisting of SEQ ID NO:5 or CNPsp(14-23) peptide having an amino acid sequence consisting of SEQ ID NO:6.
14. The kit of claim 13 , wherein the second anti-CNPsp antibody, or antigen-binding fragment thereof, is labeled.
15. The kit of claim 13 , wherein either the first or the second anti-CNPsp antibody, or antigen-binding fragment thereof, binds the CNPsp(1-13) peptide having an amino acid sequence consisting of SEQ ID NO:5.
16. The kit of claim 13 , wherein either the first or the second anti-CNPsp antibody, or antigen-binding fragment thereof, binds the CNPsp(14-23) peptide having an amino acid sequence consisting of SEQ ID NO:6.
17. The kit of claim 13 , wherein the solid matrix is a porous strip or chip.