Polyhydroxyalkanoate production method
Embodiments of the invention relate generally to processes for the production and processing of polyhydroxyalkanoates (PHA) from carbon sources. In several embodiments, PHAs are produced at high efficiencies from carbon-containing gases or materials.
1. A method for producing PHA-producing methanotrophic bacteria that preferentially express particulate methane monooxygenase (pMMO) for pMMO mediated polyhydroxyalkanoate (PHA) generation, the method comprising:
(a) contacting PHA-producing methanotrophic bacteria with a medium comprising one or more additional nutrients, and a carbon-containing gas that can be metabolized by said PHA-producing methanotrophic bacteria;
(b) reducing the concentration of oxygen and at least one other of said additional nutrients in said medium to cause at least a portion of said PHA-producing methanotrophic bacteria to convert the carbon from said carbon-containing gas into PHA through activity of pMMO, wherein at least one of said reduced nutrients is selected from the group consisting of copper, phosphorus, magnesium, nitrogen, carbon dioxide, and combinations thereof;
(c) inducing growth of said PHA-producing methanotrophic bacteria that produce PHA through activity of pMMO by increasing the concentration of one or more of said reduced nutrients in said medium to the concentration of said one or more nutrients in said medium prior to said reducing step, thereby resulting in production of PHA-producing methanotrophic bacteria that preferentially express pMMO for pMMO-mediated PHA production; and
(d) repeating steps (b) and (c) a plurality of times.
2. The method of claim 1 , wherein said carbon-containing gas comprises carbon dioxide.
3. A method for producing a culture of high efficiency polyhydroxyalkanoate (PHA)-producing methanotrophic bacteria that preferentially express particulate methane monooxygenase (pMMO) for pMMO-mediated PHA generation, the method comprising:
(a) contacting PHA-producing methanotrophic bacteria with a medium comprising one or more nutrients and a carbon-containing gas that can be metabolized by said PHA-producing methanotrophic bacteria;
(b) reducing the concentration of oxygen and at least one of said one or more nutrients in said medium to cause at least a portion of the PHA-producing methanotrophic bacteria to produce PHA through activity of pMMO, wherein at least one of said reduced nutrient is selected from the group consisting of copper, phosphorus, magnesium, nitrogen, carbon dioxide, and combinations thereof;
(c) increasing the concentration of one or more of said reduced nutrients in said medium to the concentration of said one or more nutrients in said medium prior to said reducing step to induce growth within said culture of PHA-producing methanotrophic bacteria that produce PHA through activity of pMMO; and
(d) repeating steps (b) and (c) a plurality of times until a culture of high efficiency PHA producing methanotrophic bacteria that preferentially express pMMO for pMMO-mediated PHA generation are produced.
4. The method of claim 3 , wherein said nutrient of step (b) and said nutrient of step (c) are the same nutrient.
5. The method of claim 3 , wherein said nutrient of step (b) and said nutrient of step (c) are different nutrients.
6. The method of claim 3 , wherein said carbon-containing gas comprises carbon dioxide.