MDCK-derived cell lines adapted to serum-free culture and suspension culture and method for preparing vaccine virus using the cells

Inventors: Yong Wook Park (Gyeonggi-Do, KR); Kun Se Lee (Seoul, KR); Bong-Yong Lee (Seoul, KR); Mahnhoon Park (Gyeonggi-Do, KR); Hun Kim (Gyeonggi-Do, KR); Yun-Hee Kim (Gyeonggi-Do, KR); Su-Jeen Lee (Gyeongg-Do, KR)

Assignee: SK Chemicals Co., Ltd.

C12N7/00 A61K39/12 A61K39/145 C12N5/0686 A61K39/00 A61K2039/525 A61K2039/5254 C12N2500/90 C12N2760/16134 C12N2760/16151 C12N2760/16152 C12N2760/16171 C12N2760/16234 C12N2760/16251 C12N2760/16252 C12N2760/16271

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Quick Facts

Patent No.: US 9,447,383
App. No.: 14/711,489
Granted: Sep 20, 2016
Kind: B2

External Links

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Abstract

Disclosed is a Madin-Darby canine kidney (MDCK)-derived cell line. The MDCK-derived cell line is derived from MDCK cells deposited under accession number ATCC CCL-34. The MDCK-derived cell line can be prepared by serum-free culture and suspension culture. Preferably, the MDCK-derived cell line has low or no tumorigenicity. The MDCK-derived cell line is preferably selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. Further disclosed are a culture method for growing the MDCK-derived cells and a method for producing a vaccine virus using the MDCK-derived cells.

Claims (1)

1. A MDCK-derived cell line which is MDCK Sky1023 (DSM ACC3112), MDCK Sky10234 (DSM ACC3114) or MDCK Sky3851 (DSM ACC3113).

Assignments (4)

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 18, 2018

From: SK CHEMICALS CO., LTD.

To: SK BIOSCIENCE CO., LTD.

Reel/Frame 047218/0676 →

CHANGE OF NAME Recorded Sep 20, 2018

From: SK CHEMICALS CO., LTD.

To: SK DISCOVERY CO., LTD.

Reel/Frame 047115/0818 →

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 23, 2018