IP Library Granted Patent US 10,830,766
Granted Patent B2
US 10,830,766 · App. 14/719,972 · Granted Nov 10, 2020

Fibrous substrate and method for making

Inventors: Srikanth Singamaneni (St. Louis, MO); Evan Kharasch (St. Louis, MO); Jeremiah Morrissey (St. Louis, MO); Chang Hee Lee (Tempe, AZ)
Assignee: Washington University
G01N33/54346B82Y5/00B82Y15/00B82Y20/00B82Y40/00G01N21/554G01N21/658G01N33/54373G01N33/553G01N33/57438G01N2201/02
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Quick Facts
Patent No.
US 10,830,766
App. No.
14/719,972
Granted
Nov 10, 2020
Kind
B2
Abstract

Natural and/or synthetic antibodies for specific proteins are adhered to nanoparticles. The nanoparticles are adhered to a substrate and the substrate is exposed to a sample that may contain the specific proteins. The substrates are then tested with surface enhanced Raman scattering techniques and/or localized surface plasmon resonance techniques to quantify the amount of the specific protein in the sample.

Claims (12)

1. A fibrous substrate comprising a plasmonic biosensor comprising a nanostructure core and an agent that specifically binds to a target molecule immobilized to the nanostructure core, wherein the plasmonic biosensor is adsorbed to the fibrous substrate, and wherein the nanostructure core comprises metal nanorods.

2. The substrate of claim 1 wherein the fibrous substrate is selected from the group consisting of a paper and a fibrous mat.

3. The fibrous substrate of claim 1 wherein the nanostructure core is selected from the group consisting of a gold nanostructure core, a silver nanostructure core, a copper nanostructure core, and combinations thereof.

4. The fibrous substrate of claim 1 further comprising a linker to immobilize the agent to the nanostructure core.

5. A method of preparing a fibrous substrate comprising a plasmonic biosensor, the method comprising:

preparing a plasmonic biosensor by a method comprising

synthesizing a nanostructure core, wherein the nanostructure core comprises metal nanorods; and

immobilizing at least one agent to the nanostructure core, wherein the agent specifically binds to a target molecule; and

adsorbing the plasmonic biosensor to a fibrous substrate.

6. The method of claim 5 wherein the fibrous substrate is selected from the group consisting of a paper and a fibrous mat.

7. The method of claim 5 wherein the nanostructure core is selected from the group consisting of a gold nanostructure core, a silver nanostructure core, a copper nanostructure core, and combinations thereof.

8. The method of claim 5 wherein the at least one agent is immobilized to the nanostructure core by a linker.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 22, 2015
From: SINGAMANENI, SRIKANTH; KHARASCH, EVAN; MORRISSEY, JEREMIAH; LEE, CHANG HEE
To: WASHINGTON UNIVERSITY
Reel/Frame 035700/0226 →
Continuity (4)
Division 13309852 · Dec 2, 2011
Provisional Application 61419573 · Dec 3, 2010
Provisional Application 61487441 · May 18, 2011
Related Publication 20150253317A1 · Sep 10, 2015