ANTIBODY FORMULATIONS AND METHODS
Antibody formulations and methods useful for prophylaxis or treatment of amyloidosis, including AA amyloidosis and AL amyloidosis.
1 - 33 . (canceled)
34 . A nucleic acid comprising a nucleotide sequence encoding at least one polypeptide having an amino acid sequence set forth as any one of SEQ ID NOs: 13-16.
35 . The nucleic acid of claim 34 comprising the nucleotide sequence of SEQ ID NO: 19.
36 . The nucleic acid of claim 35 comprising the nucleotide sequence of SEQ ID NO: 20.
37 - 38 . (canceled)
39 . The nucleic acid of claim 38 comprising the nucleotide sequence of SEQ ID NO: 22.
40 . The nucleic acid of claim 39 comprising the nucleotide sequence of SEQ ID NO: 23.
41 . A vector comprising at least one nucleic acid of claim 34 .
42 . The vector of claim 41 , comprising a nucleic acid comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence set forth as SEQ ID NO: 13 and a nucleic acid comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence set forth as SEQ ID NO: 15.
43 . The vector of claim 41 , comprising a nucleic acid comprising a nucleotide sequence set forth as SEQ ID NO: 19 and a nucleic acid comprising a nucleotide sequence set forth as SEQ ID NO: 22.
44 . The vector of claim 41 , comprising a nucleic acid comprising a nucleotide sequence set forth as SEQ ID NO: 20 and a nucleic acid comprising a nucleotide sequence set forth as SEQ ID NO: 23.
45 . A host cell comprising at least one nucleic acid of claim 34 stably incorporated into its genome.
46 . The host cell of claim 45 , wherein the host cell is a CHO cell.
47 . The host cell of claim 45 having stably incorporated into its genome a nucleic acid comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence set forth as SEQ ID NO: 13 and a nucleic acid comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence set forth as SEQ ID NO: 15.
48 . The host cell of claim 45 having stably incorporated into its genome a nucleic acid comprising a nucleotide sequence set forth as SEQ ID NO: 19 and a nucleic acid comprising a nucleotide sequence set forth as SEQ ID NO: 22.
49 . The host cell of claim 45 having stably incorporated into its genome a nucleic acid comprising a nucleotide sequence set forth as SEQ ID NO: 20 and a nucleic acid comprising a nucleotide sequence set forth as SEQ ID NO: 23.
50 . The host cell of claim 47 , wherein the host cell is a CHO cell.
51 . A method of making a pharmaceutical formulation comprising:
(a) culturing mammalian cells having stably incorporated into their genome one or more nucleic acids encoding a light chain and a heavy chain of a humanized 2A4 antibody so that the mammalian cells secrete the antibody into cell culture media;
(b) purifying the antibody from the cell culture media; and
(c) preparing the pharmaceutical formulation.
52 . The method of claim 51 , wherein the nucleic acid encoding the light chain of a humanized 2A4 antibody comprises a nucleotide sequence encoding a polypeptide having an amino acid sequence set forth as SEQ ID NO: 13, and wherein the nucleic acid encoding the heavy chain of a humanized 2A4 antibody comprises a nucleotide sequence encoding a polypeptide having an amino acid sequence set forth as any one of SEQ ID NOs: 14-16.
53 . The method of claim 52 , wherein the nucleic acid encoding the light chain of a humanized 2A4 antibody comprises a nucleotide sequence encoding a polypeptide having an amino acid sequence set forth as SEQ ID NO: 13, and wherein the nucleic acid encoding the heavy chain of a humanized 2A4 antibody comprises a nucleotide sequence encoding a polypeptide having an amino acid sequence set forth as SEQ ID NO: 15.
54 . The method of claim 53 , wherein the nucleic acid encoding the light chain of a humanized 2A4 antibody comprises a nucleotide sequence of SEQ ID NO: 19, and wherein the nucleic acid encoding the heavy chain of a humanized 2A4 antibody comprises a nucleotide sequence of SEQ ID NO: 22.
55 . The method of claim 54 , wherein the nucleic acid encoding the light chain of a humanized 2A4 antibody comprises a nucleotide sequence of SEQ ID NO: 20, and wherein the nucleic acid encoding the heavy chain of a humanized 2A4 antibody comprises a nucleotide sequence of SEQ ID NO: 23.
56 - 86 . (canceled)