The present invention provides, inter alia, an isolated cell line, 3M as well as methods for making such a cell line and methods of using such a cell line, e.g., to produce a protein such as an immunoglobulin.
1. An isolated Chinese hamster ovary cell deposited at the American Type Culture Collection under deposit number PTA-10481 that comprises an immunoglobulin light chain or an immunoglobulin heavy chain or both.
2. The cell of claim 1 that comprises both an immunoglobulin light chain and an immunoglobulin heavy chain.
3. The cell of claim 1 in an aqueous liquid cell culture medium.
4. The cell of claim 1 in a vessel.
5. A master cell bank or working cell bank comprising the cell of claim 1 .
6. The cell of claim 1 in a cell freezing medium.
7. The cell of claim 6 wherein the cell freezing medium comprises dimethylsulfoxide.